A Simple, High-Precision, High-Sensitivity Tracer Assay for N(inf2) Fixation.

Applied and Environmental Microbiology
J P MontoyaD G Capone

Abstract

We describe a simple, precise, and sensitive experimental protocol for direct measurement of N(inf2) fixation using the conversion of (sup15)N(inf2) to organic N. Our protocol greatly reduces the limit of detection for N(inf2) fixation by taking advantage of the high sensitivity of a modern, multiple-collector isotope ratio mass spectrometer. This instrument allowed measurement of N(inf2) fixation by natural assemblages of plankton in incubations lasting several hours in the presence of relatively low-level (ca. 10 atom%) tracer additions of (sup15)N(inf2) to the ambient pool of N(inf2). The sensitivity and precision of this tracer method are comparable to or better than those associated with the C(inf2)H(inf2) reduction assay. Data obtained in a series of experiments in the Gotland Basin of the Baltic Sea showed excellent agreement between (sup15)N(inf2) tracer and C(inf2)H(inf2) reduction measurements, with the largest discrepancies between the methods occurring at very low fixation rates. The ratio of C(inf2)H(inf2) reduced to N(inf2) fixed was 4.68 (plusmn) 0.11 (mean (plusmn) standard error, n = 39). In these experiments, the rate of C(inf2)H(inf2) reduction was relatively insensitive to assay volume. Our results, the firs...Continue Reading

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Citations

Jan 9, 2008·Rapid Communications in Mass Spectrometry : RCM·Urumu TsunogaiFumiko Nakagawa
Aug 11, 2012·Nature·Tobias GroßkopfJulie LaRoche
Aug 11, 2012·Nature·Angelicque E White
Jul 11, 2013·PloS One·Ryan J WoodlandPerran L M Cook
Dec 19, 2013·PloS One·Sophie BonnetDouglas G Capone
Aug 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianguo YangYi-Ping Wang
Oct 30, 2015·Proceedings. Biological Sciences·Ulisse CardiniChristian Wild
Feb 3, 2016·Scientific Reports·Drew MacKellarJürgen Prell
Oct 23, 2019·The ISME Journal·Isabell KlawonnHelle Ploug
Mar 1, 2008·The ISME Journal·Allison A FongMatthew J Church
Jun 21, 2011·PloS One·Camila FernandezOsvaldo Ulloa
Aug 26, 2009·Microbial Ecology·Loic CharpyStjepko Golubic
Jan 20, 2011·Environmental Microbiology·Tali YogevIlana Berman-Frank
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Christian SchlosserC Mark Moore
Dec 16, 2011·The ISME Journal·Hannah HalmMarcel M M Kuypers
Jan 24, 2015·Critical Reviews in Biotechnology·Dominique GrizeauJack Legrand
Jun 24, 2016·The Journal of Experimental Biology·Mar BenavidesSophie Bonnet
Aug 25, 2016·Scientific Reports·Katsura IgaiMasahiro Umezaki
Oct 11, 2013·Environmental Microbiology·Anne E DekasVictoria J Orphan
Jun 5, 2012·Applied and Environmental Microbiology·Babur S Mirza, Jorge L M Rodrigues

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