A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination

Malaria Journal
Sumudu BrittonJames S McCarthy

Abstract

To detect all malaria infections in elimination settings sensitive, high throughput and field deployable diagnostic tools are required. Loop-mediated isothermal amplification (LAMP) represents a possible field-applicable molecular diagnostic tool. However, current LAMP platforms are limited by their capacity for high throughput. A high-throughput LAMP (HtLAMP) platform amplifying mitochondrial targets using a 96-well microtitre plate platform, processing 85 samples and 11 controls, using hydroxynaphtholblue as a colourimetric indicator was optimized for the detection of malaria parasites. Objective confirmation of visually detectable colour change results was made using a spectrophotometer. A dilution series of laboratory-cultured 3D7 Plasmodium falciparum parasites was used to determine the limit of detection of the HtLAMP assay, using P. falciparum (HtLAMP-Pf) and Plasmodium genus (HtLAMP-Pg) primers, on whole blood and filter paper, and using different DNA extraction protocols. The diagnostic accuracy of HtLAMP was validated using clinical samples from Papua New Guinea, Malaysia, Ghana and The Gambia and its field applicability was evaluated in Kota Marudu district hospital, Sabah, Malaysia. The HtLAMP assay proved to be a s...Continue Reading

References

Oct 1, 1993·Molecular and Biochemical Parasitology·G SnounouK N Brown
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y MoriT Notomi
Oct 23, 2002·The American Journal of Tropical Medicine and Hygiene·Russell E ColemanJetsumon Sattabongkot
Jun 14, 2003·Annals of Tropical Medicine and Parasitology·D PadleyJ Saldanha
Apr 21, 2005·PLoS Medicine·Colin J SutherlandGeoffrey A T Targett
Feb 7, 2008·PLoS Neglected Tropical Diseases·Zablon Kithinji NjiruAndrew Richard Christopher Thompson
Oct 24, 2009·The Journal of Infectious Diseases·Lucy C OkellChris J Drakeley
Jun 18, 2010·Journal of Clinical Microbiology·Spencer D PolleyColin J Sutherland
Nov 10, 2010·PloS One·Naomi W LucchiVenkatachalam Udhayakumar
Nov 13, 2010·PLoS Neglected Tropical Diseases·Sally L WastlingSusan C Welburn
Feb 12, 2011·PLoS Medicine·UNKNOWN malERA Consultative Group on Diagnoses and Diagnostics
May 2, 2013·The Journal of Infectious Diseases·Spencer D PolleyColin J Sutherland
Jan 31, 2014·Journal of Clinical Microbiology·Christina M ScheelAnastasia P Litvintseva
Feb 18, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Bismarck DinkoColin J Sutherland
May 6, 2014·The Journal of Infectious Diseases·Michelle S HsiangPhilip J Rosenthal

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Citations

May 11, 2016·The American Journal of Tropical Medicine and Hygiene·Sumudu BrittonJames S McCarthy
Sep 20, 2017·Diagnostics·Francis D KrampaProsper Kanyong
Nov 11, 2017·Lab on a Chip·N KolluriM Cabodi
Oct 11, 2017·The American Journal of Tropical Medicine and Hygiene·Melissa D ConradPhilip J Rosenthal
Dec 19, 2017·Parasite : Journal De La Société Française De Parasitologie·Camille PonceStéphane Picot
Feb 9, 2019·Medical and Veterinary Entomology·C D SchenkelC Silaghi
Feb 6, 2019·European Journal of Microbiology & Immunology·Hans KollendaHagen Frickmann
Oct 3, 2019·Biosensors·Faye M Walker, Kuangwen Hsieh
Dec 5, 2018·Chemical Reviews·Christine F MarkwalterDavid W Wright

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Methods Mentioned

BETA
PCR
electrophoresis
Assay

Software Mentioned

Pf
LAMP
PowerwaveXS2
malERA
HtLAMP

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