A simple human dermal model for assessment of in vitro attachment efficiency of stored cultured epithelial autografts

The Journal of Burn Care & Rehabilitation
M M GhoshS MacNeil

Abstract

This study investigated the effect of short-term storage on the viability and in vitro attachment efficiency of cultured epithelial autograft sheets. Four storage protocols were investigated: overnight at 37 degrees C in keratinocyte culture medium, overnight at 4 degrees C in phosphate-buffered saline solution, overnight at -80 degrees C in cryopreservation medium (containing 10% dimethyl sulphoxide), and 1 week at -80 degrees C in cryopreservation medium. Viability was assessed before and after storage by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. All the storage conditions significantly reduced viability compared with fresh sheets, and no significant decrease was seen when the sheets stored under the different protocols were compared with each other. The best viability obtained was 60% of that of the fresh sheets. The in vitro viability of these stored sheets was then compared with that of the fresh sheets by culturing them on deepidermized acellular allodermis and assessing the composites formed by light microscopy and the MTT assay. The fresh sheets attached and formed a histologically demonstrable composite with the dermal substrate, whereas none of the stored sheets formed demonstrable ...Continue Reading

Citations

Mar 1, 1996·Burns : Journal of the International Society for Burn Injuries·R A DawsonS MacNeil
Mar 6, 2012·Burns : Journal of the International Society for Burn Injuries·Zhe LiPeter Kennedy
Nov 9, 2005·Burns : Journal of the International Society for Burn Injuries·Bishara S AtiyehS William Gunn
Oct 27, 2010·Tissue Engineering. Part C, Methods·Paula C EvesSheila Mac Neil
Jan 18, 2005·World Journal of Surgery·Bishara S AtiyehShady N Hayek

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