A simple in situ cell-based sumoylation assay with potential application to drug screening

Bioscience, Biotechnology, and Biochemistry
Miyuki MuramatsuHisato Saitoh

Abstract

We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.

References

Jan 17, 2002·Cell·Andrea PichlerFrauke Melchior
May 12, 2006·Experimental Cell Research·Noriko SaitohMitsuyoshi Nakao
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Mar 7, 2009·The Journal of Antibiotics·Isao FukudaMinoru Yoshida

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Citations

Aug 9, 2013·Bioscience, Biotechnology, and Biochemistry·Shinpei ObataHisato Saitoh
Jul 9, 2013·Bioscience, Biotechnology, and Biochemistry·Ayumi Kanemaru, Hisato Saitoh
Jul 20, 2012·Biochemistry Research International·Elisa Da Silva-FerradaRune Matthiesen
Oct 13, 2017·Open Biology·Yanfang YangShufang Liang

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