A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels

Biofabrication
Hyojin KoAli Khademhosseini

Abstract

Physicochemical and biological gradients are desirable features for hydrogels to enhance their relevance to biological environments for three-dimensional (3D) cell culture. Therefore, simple and efficient techniques to generate chemical, physical and biological gradients within hydrogels are highly desirable. This work demonstrates a technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels by stacking and crosslinking prehydrogel solution in a layer by layer manner. Partial crosslinking of the hydrogel allows mixing of prehydrogel solution with the previous hydrogel layer, which makes a smooth gradient profile, rather than discrete layers. This technique enables the generation of concentration gradients of bovine serum albumin in both gelatin methacryloyl (GelMA) and poly(ethylene glycol) diacrylate hydrogels, as well as mechanical gradients across a hydrogel containing varying gel concentrations. Fluorescence microscopy, mechanical testing, and scanning electron microscopy show that the gradient profiles can be controlled by changing both the volume and concentration of each layer as well as intensity of UV exposure. GelMA hydrogel gradients with different Young's moduli were successfully use...Continue Reading

References

Jul 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Jason A BurdickRobert Langer
Apr 19, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Rico C GunawanDeborah E Leckband
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Dec 21, 2007·Lab on a Chip·Thomas M Keenan, Albert Folch
Sep 23, 2008·Tissue Engineering. Part B, Reviews·Milind SinghMichael S Detamore
Jun 27, 2009·Science·Dennis E DischerPeter W Zandstra
Apr 28, 2010·Cell Adhesion & Migration·Ciara M Murphy, Fergal J O'Brien
Aug 6, 2010·Biomaterials·Katrin BottSimone C Rizzi
Oct 1, 2010·Advanced Materials·Brandon V SlaughterNicholas A Peppas
Jun 21, 2011·Biomaterials·Matthew J HancockAli Khademhosseini
Aug 30, 2011·The Canadian Journal of Chemical Engineering·Shilpa SantAli Khademhosseini
Oct 29, 2011·Trends in Cell Biology·Dongeun HuhDonald E Ingber
Jan 31, 2012·Acta Biomaterialia·Iris Mironi-HarpazDror Seliktar
Mar 25, 2014·Colloids and Surfaces. B, Biointerfaces·Jindan WuChangyou Gao
Sep 30, 2014·Colloids and Surfaces. B, Biointerfaces·Pian WuKaiyong Cai
Apr 15, 2015·Frontiers in Bioengineering and Biotechnology·Sébastien G RicoultDavid Juncker
Apr 17, 2015·Advanced Healthcare Materials·Xin ZhaoAli Khademhosseini
Apr 29, 2016·Nature Methods·Steven R Caliari, Jason A Burdick
Sep 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Christopher D HartmanJoyce Y Wong
May 6, 2017·Science·Yu Shrike Zhang, Ali Khademhosseini
Aug 31, 2017·Nature Communications·Britta TrappmannChristopher S Chen
Apr 11, 2018·Biomolecules & Therapeutics·Ji Hyeon KimHyun-Jung Kim

❮ Previous
Next ❯

Citations

Apr 25, 2020·Polymers·Natalia ZinkovskaMiloslav Pekar
Jul 12, 2020·Trends in Biotechnology·Chunching LiMolly M Stevens
Jan 19, 2021·Advanced Functional Materials·Jeremy M Lowen, J Kent Leach
Dec 24, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Vicki VaniaGuixue Wang
Sep 21, 2021·Tissue Engineering. Part C, Methods·Melanie KahlChristoph Meinert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.