A simple method for determining metronidazole resistance of Helicobacter pylori.

Journal of Clinical Microbiology
T H HenriksenA Lia

Abstract

The reliability of methods for determination of Helicobacter pylori resistance to metronidazole has been found to depend upon the incubation time. Because the disk diffusion method is more vulnerable than other methods to prolonged incubation, this method has not been recommended for H. pylori. However, because media designed for rapid growth of H. pylori have been introduced, the time has come to look at the clinical usefulness of this inexpensive and simple method again. The correlation of readings obtained with the E test (AB Biodisk, Solna, Sweden) and Rosco's (Taastrup, Denmark) disk diffusion method for in vitro metronidazole resistance determination for H. pylori with a short incubation time (24 to 31 h) was studied. Plates which could not be read after 24 to 31 h were reincubated for another night. Fifty-seven consecutive clinical strains were tested. Because the rate of regrowth of H. pylori depends upon the age of the colonies inoculated, the reproducibility of resistance test results for young colonies versus old colonies was also studied. Resistance plates could be read after 24 to 31 h of incubation for 28 of 29 strains when the inoculum consisted of young colonies (3 to 4 days old). For these 29 strains, a high co...Continue Reading

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Citations

Jan 13, 2000·Alimentary Pharmacology & Therapeutics·E J Van Der WoudenJ H Kleibeuker
Jun 27, 2000·Australian and New Zealand Journal of Medicine·A G FraserB Hollis
Mar 23, 2002·The Journal of Antimicrobial Chemotherapy·Cliodna McNultyUNKNOWN PHLS Helicobacter Working Group

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