A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene

Legal Medicine
Claudio M BraviGuillermo Giovambattista

Abstract

We developed a simple, quick assay in order to discriminate forensic samples among human, and common domestic and livestock species of the Pampean region, Argentina. A mitochondrial cytochrome b fragment amplified with universal primers was separately digested with three restriction enzymes (AluI, HaeIII, and HinfI) and the resulting fragments were resolved through electrophoresis in polyacrylamide gels. This PCR-RFLP method allowed us to identify the target species and worked on degraded samples. The assay was successfully applied in livestock robbery cases in Argentine, and may be useful when attempting a first assessment as to the specific status of a forensic evidence.

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Citations

Jul 18, 2006·International Journal of Legal Medicine·Takashi KitanoMotoki Osawa
Dec 5, 2008·International Journal of Legal Medicine·Hiroaki NakamuraIsao Yuasa
Dec 1, 2009·Forensic Science, Medicine, and Pathology·Yasser Said El-SayedSalah M Abd El-Rahman
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Aug 19, 2009·Journal of Forensic Sciences·P R MeganathanIkramul Haque
Jul 18, 2008·Journal of Forensic Sciences·Silvina DíazGuillermo Giovambattista
Apr 14, 2010·Journal of Forensic Sciences·Bhawna DubeyIkramul Haque
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Nov 7, 2019·Journal of Food Science and Technology·Chihiro MatsumotoHiroyuki Kawachi
Aug 13, 2020·International Journal of Legal Medicine·Mariana RoccaroAngelo Peli
Jun 21, 2020·International Journal of Legal Medicine·Chikahiro Mori, Shuichi Matsumura
Oct 10, 2021·International Journal of Legal Medicine·Chikahiro Mori, Shuichi Matsumura

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