PMID: 7086153Jan 1, 1982Paper

A simple method for electrophoretic analysis of cell surface glycoproteins based on concanavalin A binding

Journal of Immunological Methods
T Krakauer, R D Camerini-Otero

Abstract

A simple and sensitive method is described for the detection of cell surface proteins. This method consists of reacting concanavalin A (Con A) with 35S-labeled methionine, detergent solubilized whole cell extracts and immunoprecipitating the Con A-protein complexes with anti-Con A antibody bound to Staphylococcus aureus Cowan I strain (SACI). These reagents are commercially available, stable and easy to standardize. Mouse lymphocytes cell surface proteins purified by this procedure show great similarities to those purified with goat anti-cell surface antibodies.

References

Nov 1, 1977·The Journal of Experimental Medicine·P P Jones
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Burridge
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
May 26, 1972·Biochemical and Biophysical Research Communications·M J Hayman, M J Crumpton

Citations

Jul 15, 1986·Virology·M del ValE Viñuela
Mar 1, 1987·Journal of Veterinary Pharmacology and Therapeutics·G E BurrowsB Martin

Related Concepts

Metazoa
Electrophoresis, Agar Gel
Neoglycoproteins
Lymphoid Cells
Cell Surface Proteins
Mice, Inbred C57BL
Receptors, Concanavalin A
Mouse, Swiss

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