A simple method for evaluating Cryptosporidium-specific antibodies used in monitoring environmental water samples

Letters in Applied Microbiology
G VeseyD A Veal

Abstract

A simple method is described for the evaluation and quality control of Cryptosporidium-specific antibodies used in monitoring environmental water samples. Purified oocysts were fluorescently labelled with a test antibody at the appropriate concentration. Labelled oocysts were analysed using flow cytometry and a region was defined on a bivariate dotplot of fluorescence versus light scatter that enclosed all oocysts. Concentrates of environmental water samples that did not contain oocysts were then incubated with the test antibody and analysed using flow cytometry. The number of particles that appeared in the region defined for oocysts was recorded and was a measure of non-specific binding. The technique provides a simple, rapid and quantitative tool for both evaluating the binding specificity of test antibodies and optimizing sample staining conditions.

Citations

Jun 4, 2005·Environmental Monitoring and Assessment·Bing-Mu HsuChien-Min Kung
Sep 15, 2000·Journal of Immunological Methods·D A VealP V Attfield
Feb 22, 2005·Journal of Microbiological Methods·Aurélien Dumètre, Marie-Laure Dardé
Jan 24, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Belinda C Ferrari, Duncan Veal
Sep 5, 2008·Current Protocols in Cytometry·Jonathan Porter
Oct 20, 1999·Canadian Journal of Microbiology·C E Höglund, T A Stenström
Sep 6, 2000·Clinical and Diagnostic Laboratory Immunology·C WeirK Williams
Feb 7, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·B C Ferrari, P L Bergquist
Feb 7, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michel MontemayorFrancisco Lucena
Jun 29, 1999·Analytical Chemistry·S D Richardson

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