A simple method for histopathological evaluation of organoids

Journal of Toxicologic Pathology
Etsuko FujiiMasami Suzuki

Abstract

In vitro-cultured 3D structures called organoids have become important tools for biological research, but there is little information concerning simple and efficient methods to evaluate organoid morphology. To address this issue, we attempted to establish a simple method by applying conventional histopathology that enables observation of multiple organoids on a single cross section, maintains good morphology, and is applicable to various histopathological stains. By centrifugation in unsolidified agarose solution, we were able to accumulate the organoids onto a single plane. The morphology was well preserved, and the various morphological types and sizes of organoid structures were identified. This method was also applicable for special staining, immunohistochemistry, and immunofluorescence staining. This method makes it possible to utilize the advantages of conventional pathological methods when studying organoids.

References

Jul 19, 2014·Science·Madeline A Lancaster, Juergen A Knoblich
Feb 24, 2015·Frontiers in Medicine·Yuki Ohta, Toshiro Sato
Mar 20, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Katia CarneiroMaria I D Rossi
Oct 26, 2016·Stem Cells·Gaurav KaushikAli Khademhosseini
Mar 16, 2017·Development·Jarno Drost, Hans Clevers

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Citations

Jul 31, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Masaki YamazakiMasami Suzuki
Jun 6, 2020·Biomedical Optics Express·Peter F FavreauMelissa C Skala
Sep 24, 2021·Journal of Tissue Engineering and Regenerative Medicine·Shi-Wei ZhangChang-Hua Zhang

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