PMID: 7014723Jan 1, 1981Paper

A simple method for isolating specific antibodies to complement components

Journal of Immunological Methods
S Bhakdi, W Kayser

Abstract

A simple procedure for isolating specific antibodies to complement components C3, C4 and C5b-9 from whole rabbit antisera or immunoglobin preparations is described. Antisera are first reacted with complement-treated sheep erythrocyte membranes. Immunoglobulins are eluted from the washed membranes with acetic acid at pH 2.4, and further purified by one absorption/desorption step on Protein A Sepharose. The final protein preparations contain greater than 90% rabbit immunoglobins, as assessed by quantitative immunoelectrophoresis and SDS polyacrylamide gel electrophoresis. Binding assays with radioiodinated protein preparations indicate a content of specific antibodies in the range 5-55% of total protein. Since there is virtually no non-specific binding of protein to control cells or membranes, the immunoglobulin preparations are well suited for use in sensitive immunoassays for complement components.

References

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W P Kolb, H J Müller-Eberhard
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Bhakdi, J Tranum-Jensen
Jan 1, 1978·Journal of Immunological Methods·C Mueller-EckhardtI Mahn
Jul 1, 1978·Transfusion·D E JenkinsW H Moore
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N H Axelsen
Jul 5, 1958·Nature·A S McFARLANE

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Citations

Feb 25, 1983·Journal of Immunological Methods·S Bhakdi, M Muhly
Oct 1, 1981·The Journal of Cell Biology·R FüssleH J Wellensiek
Jul 1, 1983·British Journal of Haematology·W KayserK Ebert
Sep 1, 1986·Kidney International·N HinglaisJ Bariety
Aug 11, 1983·Biochimica Et Biophysica Acta·S Bhakdi, J Tranum-Jensen

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