A simple method for measuring the effects of drugs on intestinal longitudinal and circular muscle

Journal of Pharmacological and Toxicological Methods
I M Coupar, L Liu

Abstract

A tissue preparation is described which makes it possible to examine the effect of drugs on the nerves that control both longitudinal and circular muscles of the small intestine. The preparation consists of a segment of rat ileum set up to measure the tension developed in the longitudinal muscle and intraluminal pressure developed by the circular muscle in response to transmural electrical stimulation. The method showed that both muscles differed in their patterns of responses to transmural stimulation from the threshold of 2.5-60 Hz. All responses were tetrodotoxin-sensitive, the fast longitudinal muscle contraction and the intraluminal pressure response being caused by both pre- and postganglionic cholinergic nerves. The responses differed in their sensitivities to the alpha 2-adrenoceptor agonists clonidine and UK 14,304, whereby the cholinergic responses of the circular but not longitudinal muscle were inhibited by the agonists. Comparison of results with the guinea-pig ileum set up in the same way revealed a marked species variation in responses, in that the intraluminal pressure response of the guinea-pig ileum was not sensitive to alpha 2-adrenoceptor agonists. This clear species difference makes it possible to use the c...Continue Reading

References

Mar 3, 1992·European Journal of Pharmacology·F MulèR Serio
Aug 1, 1988·British Journal of Pharmacology·I Muramatsu, N Yanaihara
Oct 1, 1989·British Journal of Pharmacology·R M EglenR L Whiting
Jul 1, 1971·The Journal of Physiology·W D PatonM A Zar
Feb 1, 1969·The Journal of Physiology·S R Kottegoda
Mar 6, 1995·European Journal of Pharmacology·F MulèA Postorino
Mar 1, 1996·British Journal of Pharmacology·L Liu, I M Coupar
Jan 1, 1963·The Journal of Physiology·W D PATON, J R VANE
Dec 1, 1963·British Journal of Pharmacology and Chemotherapy·G BROWNLEE, J HARRY
Dec 1, 1958·British Journal of Pharmacology and Chemotherapy·E BülbringO B Saxby

❮ Previous
Next ❯

Citations

Oct 2, 2015·Journal of Neurogastroenterology and Motility·Tae Sik SungIl-Suk Yang
Jun 3, 2004·Journal of Pharmacological and Toxicological Methods·A P HarrisonM A Unmack
May 13, 2014·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C KeatingD Grundy
Jan 6, 2005·World Journal of Gastroenterology : WJG·Jun-Ho LaIl-Suk Yang
Oct 9, 2019·Best Practice & Research. Clinical Gastroenterology·Ahmad Al-SaffarTakafumi Sakai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Related Papers

European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
H IshiiJ Utsunomiya
Indian Journal of Pediatrics
S K YachhaS S Baijal
© 2021 Meta ULC. All rights reserved