PMID: 6969213Jan 1, 1980Paper

A simple method for specific depletion of B lymphocytes from bovine peripheral blood mononuclear cells

Immunological Communications
W R Usinger, G A Splitter

Abstract

The treatment of bovine peripheral blood mononuclear cells with rabbit anti-bovine immunoglobulin, goat anti-rabbit immunoglobulin (GAR) and complement resulted in the specific lysis of all surface immunoglobulin (SIg) bearing B lymphocytes. No SIg+ lymphocytes were detected after 12 hours of culture following lytic treatment, whereas cells treated with antibody without complement readily stained for SIg. The percentage of cells forming E-rosettes or binding peanut agglutinin (PNA) increased following lysis. The percentage of latex-ingesting monocytes also increased after lysis even though many of these cells had cytophilic Ig. B lymphocyte-depleted (T cell-enriched) populations cultured with phytohemagglutinin (PHA) and concanavalin A (Con A) were never less reactive than unseparated cells. No differences in background mitosis was observed for these 2 cells preparations. These results suggest that bovine SIg- cells do not require B lymphocytes to respond to PHA and Con A and that lysis of B lymphocytes does not alter the responsiveness of the recovered SIg- cells.

References

Jan 1, 1979·Immunological Communications·J W Chiao, R A Good
Jan 1, 1979·Journal of Immunological Methods·T W PearsonK S Mayor-Withey
Jan 1, 1976·American Journal of Hematology·M C TerritoM J Cline
Jan 1, 1971·The Journal of Experimental Medicine·E RabellinoE R Unanue

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