Jan 1, 1976

A simple method for the cryopreservation of lymphocytes. Retention of specific immune effector functions by frozen-stored cells

Clinical and Experimental Immunology
C K Grant

Abstract

A simple, quick and inexpensive method for cryostorage of lymphocytes is discribed. When injected into appropriate normal recipients, frozen-stored rat and sheep lymphocytes caused GVH and NLT reactions respectively. Sheep lymphocytes remained viable after several months storage and functioned satisfactorily as 51Cr-labelled target cells in assays for cytotoxic antibodies. Lymphocytes, including immunoblasts, drained from sheep efferent lymphatics during immune responses to injected murine P815 cells or allogeneic lymphocytes survived freezing; when thawed the cells retained specific immune cytotoxic effector functions including the ability to secrete complement-dependent and leucocyte-dependent antibodies

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Mentioned in this Paper

Immune Response
Blood Preservation
Allogeneic lymphocytes
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Murine
Set of Efferent Lymphatic Vessels
Lymphoid Cells
Immunoblast Count
Dall Sheep
Graft Vs Host Reaction

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