PMID: 9187716Jun 1, 1997Paper

A simple method for the isolation and culture of epithelial and stromal cells from benign and neoplastic prostates

Urology
D KrillS C Strom

Abstract

Current primary prostate cell culture techniques use an overnight digestion or extensive media preparation. In this report, we describe a method for the culture of benign and neoplastic cells from human prostatectomy specimens that is rapid and contains no undefined factors in the medium. Characterization of the human cultured prostate cells was performed using immunohistochemical methods and monoclonal antibodies AE1/AE3 and cytokeratin 8, as well as monoclonal antibodies against prostate-specific antigen (PSA). Polymerase chain reaction was used to measure the exclusive epithelial and stromal cell products, c-met and hepatocyte growth factor (HGF), respectively. Electron microscopy was performed to assess the cell junctions and morphologic features of epithelial cells. Optimum cell growth in different media was tested using a cell replication assay. Microscopic evidence revealed that the cells demonstrate typical epithelial morphology, with polyhedral cells forming tight junctions in a continuous monolayer. Desmosomes were present in electron micrographs of epithelial cells. The cultured epithelial cells described in this report also demonstrate positive cytokeratin staining. The epithelial cells reacted positively with PSA a...Continue Reading

References

Jan 1, 1990·The Prostate·H B Carter, D S Coffey
Aug 1, 1991·The Journal of Clinical Endocrinology and Metabolism·P CohenR G Rosenfeld
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H C Isom, I Georgoff
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Citations

Oct 12, 2000·The Prostate·D M Peehl, R G Sellers
Oct 12, 2000·The Prostate·A T WeeraratnaJ T Isaacs
May 7, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Nazita YousefiehRichard P Ciavarra
Mar 21, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Alejandro A HidalgoSergio A Onate
May 25, 2002·In Vitro Cellular & Developmental Biology. Animal·Antonio A SinisiDonatella Tramontano
Dec 8, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takeshi SasakiYoshiki Sugimura
Oct 21, 2003·International Journal of Cancer. Journal International Du Cancer·Kenneth D SomersRichard P Ciavarra
Feb 19, 2010·The Prostate·Marie-Claude BattistaNicole Gallo-Payet
Mar 2, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Hidenori Kawashima, Tatsuya Nakatani
Sep 16, 2004·Journal of Cellular Biochemistry·Meral GuzeyRobert H Getzenberg
Dec 24, 2002·Prostate Cancer and Prostatic Diseases·F L MartinD H Phillips
Nov 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amit GuptaShahrokh F Shariat

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