Jul 1, 1989

A simple method for the morphological analysis of human ova: zona penetration in oocytes failing to fertilize or fertilizing abnormally in vitro

Human Reproduction
G M Hartshorne

Abstract

A simple and rapid technique is described whereby several unfertilized oocytes and embryos can be processed simultaneously for embedding and sectioning for histological analysis. Oocytes are encased in a serum matrix and thereafter can be handled with ease. A total of 112 unfertilized oocytes and abnormal embryos following insemination in vitro have been examined. Only 31% of oocytes showed pyknotic nuclei after a mean of 4.0 +/- 1.24 days in culture and 37% had recognizable metaphase II chromosomes. Twenty per cent showed evidence of fertilization, but spermatozoa had failed entirely to penetrate the zonae pellucidae of 34% of oocytes. The mean percentages of penetrating sperm were not significantly different between oocytes which did and did not fertilize. Under these in-vitro conditions, approximately 1-2% of spermatozoa reaching the oocyte penetrated into the zona pellucida and a lesser number to the perivitelline space. Polar bodies were also observed and the incidence of divisions, retention and parthenogenesis estimated. This technique may advance our understanding of the reasons for the success or failure of fertilization in vitro.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Fertility
Embryo
Polar Bodies
Ovum
Histology Procedure
Sectioning Technique
Cell Division
Mitotic Metaphase
Sperm Cell
Spermatozoa Cell Count

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.