PMID: 1200979Dec 1, 1975Paper

A simple method for the quantitation of isozyme patterns

Biochemical Genetics
R J Klebe

Abstract

Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S = K/T, where K is 1.6 X 10(-3) units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U = K (2)n-1, where n is the number of the slot producing the last visible band.

References

Oct 1, 1969·The Journal of Experimental Zoology·C L Markert, Y Masui
Oct 1, 1967·The Journal of Experimental Zoology·F H Ruddle, L Harrington
May 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·C L Markert, F Møller

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Citations

Aug 1, 1981·Biochemical Genetics·R J KlebeL Teng
Dec 1, 1977·Biochemical Genetics·S L Naylor, R J Klebe
Oct 1, 1981·Biochemical Genetics·R J KlebeC R Link
Jan 1, 1981·Immunogenetics·J H Nadeau, J Klein
Apr 12, 1979·Journal of Molecular Evolution·S D Ferris, G S Whitt
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z L CoppesA R Schwantes
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z L CoppesA R Schwantes
Aug 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z Coppes
Aug 1, 1981·Genetical Research·D S FalconerD A Williams
Dec 1, 1988·Biochemical Genetics·D G YardleyB L Hughes
Aug 14, 2003·Brazilian Journal of Biology = Revista Brasleira De Biologia·M R Aquino-SilvaA R Schwantes
May 15, 1977·Analytical Biochemistry·M E HodesE Gelb
Aug 1, 1978·Experimental Cell Research·R J KlebeW D Dickey
Dec 1, 1980·Journal of Molecular Evolution·W J Dickinson
Feb 27, 2003·The Journal of Experimental Biology·Brandon A Sheafor

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