A simple method for the subnanomolar quantitation of seven ophthalmic drugs in the rabbit eye

Analytical and Bioanalytical Chemistry
Pierre-Luc Latreille, Xavier Banquy

Abstract

This study describes the development and validation of a new liquid chromatography-tandem mass spectrometry (MS/MS) method capable of simultaneous quantitation of seven ophthalmic drugs-pilocarpine, lidocaine, atropine, proparacaine, timolol, prednisolone, and triamcinolone acetonide-within regions of the rabbit eye. The complete validation of the method was performed using an Agilent 1100 series high-performance liquid chromatography system coupled to a 4000 QTRAP MS/MS detector in positive TurboIonSpray mode with pooled drug solutions. The method sensitivity, evaluated by the lower limit of quantitation in two simulated matrices, yielded lower limits of quantitation of 0.25 nmol L(-1) for most of the drugs. The precision in the low, medium, and high ranges of the calibration curves, the freeze-thaw stability over 1 month, the intraday precision, and the interday precision were all within a 15% limit. The method was used to quantitate the different drugs in the cornea, aqueous humor, vitreous humor, and remaining eye tissues of the rabbit eye. It was validated to a concentration of up to 1.36 ng/g in humors and 5.43 ng/g in tissues. The unprecedented low detection limit of the present method and its ease of implementation allo...Continue Reading

References

Apr 1, 1973·Journal of Pharmaceutical Sciences·L H Block, R N Patel
Dec 1, 1994·The British Journal of Ophthalmology·T H Williamson, A Harris
Aug 1, 1994·Acta Ophthalmologica·R J Uusitalo, A Palkama
May 1, 1994·The British Journal of Ophthalmology·T H Williamson
Jan 30, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cara PolsonRussell Grant
Dec 31, 2005·Nucleic Acids Research·David S WishartJennifer Woolsey
Mar 2, 2006·Expert Opinion on Drug Delivery·Tsutomu YasukawaYasuhiko Tabata
Apr 18, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shawn M R Stanley, Hsiao Ching Foo
Dec 16, 2006·Biomaterials·Yolanda DieboldMaría J Alonso
Dec 1, 2007·Nucleic Acids Research·David S WishartMurtaza Hassanali
May 7, 2008·Biochemistry. Biokhimii︠a︡·R Sariri, H Ghafoori
Sep 23, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chadi AbbaraBertrand Diquet
Jan 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rajendra S Kadam, Uday B Kompella
Feb 25, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ileana A IonitaFatemeh Akhlaghi
May 9, 2009·Journal of Chromatography. a·George KaklamanosThemistoklis Dabalis
Oct 27, 2009·Analytical and Bioanalytical Chemistry·Harald JohnHorst Thiermann
Nov 10, 2010·Nucleic Acids Research·Craig KnoxDavid S Wishart
Feb 8, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shuang-Qing Zhang
Dec 24, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·E ter WeijdenF F T Ververs
Apr 18, 2012·Macromolecular Bioscience·Shengyan LiuFrank X Gu
Nov 17, 2012·Drug Development and Industrial Pharmacy·Djamila AchouriVéronique Andrieu
Oct 8, 2013·Biomaterials·Joseph B CiolinoDaniel S Kohane
Nov 10, 2013·Nucleic Acids Research·Vivian LawDavid S Wishart
Jul 18, 2014·European Journal of Drug Metabolism and Pharmacokinetics·Bing LiuLinjun You

❮ Previous
Next ❯

Citations

Feb 10, 2021·Journal of Clinical Medicine·Henning AustermannSven Schnichels
Feb 13, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pedro Henrique Reis da SilvaChristian Fernandes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.