PMID: 2493106Feb 1, 1989Paper

A simple method of canine pancreatic islet isolation and intrahepatic transplantation

The Journal of Surgical Research
J A van der VlietD E Sutherland

Abstract

Clinical pancreatic islet transplantation has been impeded by the inability to isolate an adequate mass of functional tissue that will ameliorate diabetes. A simplified method of canine islet isolation was developed that allowed for either intrasplenic or intrahepatic transplantation. Following total pancreatectomy, parenchymal digestion was accomplished by intraductal collagenase perfusion and stationary incubation. The digested tissue was dispersed by filtration through a steel mesh (400 microns), washed, and separated on a discontinuous dextran density gradient. Enhanced islet tissue (2-4 ml) was recovered from the uppermost interface of the gradient and autotransplanted. The islet isolation procedure was tested in two series of dogs undergoing either intrasplenic or intrahepatic engraftment. Immediate and sustained normoglycemia (plasma glucose less than 200 mg%) was obtained in 5 of 8 dogs (63%) in the intrasplenic group and 6 of 8 dogs (75%) in the intrahepatic group. The mean fasting plasma glucose concentration 2 weeks after transplantation was 102.8 +/- 6.4 mg% in the intrasplenic group and 103.3 +/- 8.4 mg% in the intraportal group. The mean IVGTT K-values 2 weeks after transplantation were -1.41 +/- 0.35% and -1.21 +...Continue Reading

References

Jan 1, 1977·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·V Mirkovitch, M Campiche
Oct 1, 1986·Clinical Chemistry·P E Lacy
Nov 1, 1986·The Journal of Clinical Investigation·R AlejandroD H Mintz
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Apr 1, 1987·Transplantation·D AldersonD W Scharp
May 1, 1969·Journal of Dental Research·A D JamiesonR C Caldwell
May 1, 1981·Diabetes·A Horaguchi, R C Merrell
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Sep 1, 1984·The Journal of Surgical Research·L F Cobb, R C Merrell
Feb 1, 1964·The Journal of Clinical Endocrinology and Metabolism·J A MOORHOUSEN J ROSEN

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