PMID: 12609726Mar 1, 2003

A simple micro-growth assay for enumerating bacteria

Journal of Microbiological Methods
Jeffrey D Brewster

Abstract

A simple method for nonspecific determination of bacteria concentrations in a variety of liquid samples was developed. The assay was based on the time required for a sample grown in liquid media to reach a threshold turbidity. Samples were combined with media in a covered 96-well microwell plate and the turbidity was monitored in real time as the bacteria grew in a temperature-controlled plate reader. A significant problem with growth in microwells was condensation on the cover, which prevented accurate turbidity measurement. This problem was overcome by coating the cover with a small amount of surface-active agent. Salmonella and E. coli concentrations could be determined with a relative error of approximately 20% at levels from 10 to 10(6) cells/ml (eight replicates). An assay of 10 samples with standards required 10 min to set up and 20 min for data processing using a computer spreadsheet program. Growth time at 37 degrees C ranged from 4 h for samples at 10(7) cells/ml to 16 h for samples at 10 cells/ml.

Citations

Mar 16, 2013·Journal of Industrial Microbiology & Biotechnology·Yanhong LiuLihan Huang
Sep 23, 2003·Journal of Microbiological Methods·Jeffrey D Brewster
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Bryan EricksenRobert I Lehrer
Jan 5, 2006·Applied and Environmental Microbiology·Willm Martens-Habbena, Henrik Sass
Jun 16, 2009·BMC Microbiology·W David JamiesonPaul M Orwin
Mar 14, 2012·PLoS Pathogens·Lucy J HathawayKathrin Mühlemann
Mar 1, 2012·Journal of Microscopy·D VanheckeD Studer
Mar 20, 2008·Chembiochem : a European Journal of Chemical Biology·Shouping LiuRoger W Beuerman
Nov 11, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·N ProninaM Krichevskaya
Jul 6, 2014·Journal of Food Protection·T P Oscar
Mar 28, 2018·BMC Microbiology·Fauzy NasherLucy J Hathaway
Dec 13, 2007·BioTechniques·Lydie Maresová, Hana Sychrová
Jan 31, 2018·Scientific Reports·Kosuke FujishimaLynn J Rothschild
Sep 26, 2019·Frontiers in Cellular and Infection Microbiology·Fauzy NasherLucy J Hathaway
Mar 3, 2018·Frontiers in Cellular and Infection Microbiology·Stefanie HoffmannRoman G Gerlach
Jan 22, 2020·BMC Microbiology·Annelies MüllerLucy J Hathaway
Nov 16, 2016·The Journal of Dairy Research·Catherine JeanGilles Robitaille
Dec 21, 2018·Frontiers in Microbiology·Fauzy NasherLucy J Hathaway
Jan 13, 2021·PloS One·Venkata Rao KrishnamurthiYong Wang

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