PMID: 9448038Feb 3, 1998Paper

A simple perfusion technique for isolation of maternal intervillous blood mononuclear cells from human placentae

Journal of Immunological Methods
J M MooreVenkatachalam Udhayakumar

Abstract

A noninvasive perfusion method for the recovery of maternal placental (intervillous) blood for use in immunologic assays is described. 60% of the perfused blood samples tested for fetal red blood cell (RBC) contamination were found to be pure maternal blood; in the remainder, fetal RBC contamination, with a single exception, was less than 6%. The intervillous mononuclear cells (IVBMC) isolated from this blood were of predominantly maternal origin as demonstrated by a polymerase chain reaction-based DNA typing technique. The number of IVBMC obtained was within the range of 9 to 55 X 10(6) cells. Phenotypic analysis of IVBMC surface antigens revealed that 61% of the cells were CD3 + T-cells and 18% were CD19 + B-cells. The CD4 + and CD8 + T-lymphocyte subsets accounted for 28 and 26% of the IVBMC, respectively. The IVBMC were functionally competent as evidenced by in vitro lymphoproliferation and cytokine production in response to mitogen and PPD stimulation. This technique allows for rapid and safe isolation of large numbers of IVBMC which are functionally active up to 12 h post-delivery, thus representing a significant improvement over previously described methods. It should facilitate more vigorous research in the study of ute...Continue Reading

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Citations

Nov 18, 2005·The Journal of Perinatal & Neonatal Nursing·Shyang-Yun Pamela K Shiao
Nov 19, 2003·Biochemical and Biophysical Research Communications·Douglas J PerkinsAltaf A Lal
Dec 16, 2003·American Journal of Reproductive Immunology : AJRI·Florian M CorvinusUdo R Markert
Sep 24, 1998·Lancet·J M MooreV Udhayakumar
Apr 26, 2006·Clinical and Experimental Immunology·E RindsjöA Scheynius
Sep 22, 2006·Hemoglobin·Shyang-Yun Pamela K Shiao, Ching-Nan Ou
Jan 18, 2018·The American Journal of Tropical Medicine and Hygiene·Andreea WaltmannSteven R Meshnick
May 10, 2006·Clinical and Vaccine Immunology : CVI·Caroline OthoroYa Ping Shi
May 24, 2005·Infection and Immunity·Sujittra ChaisavaneeyakornVenkatachalam Udhayakumar

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