PMID: 6409845Jun 1, 1983Paper

A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope

The Histochemical Journal
G R NewmanE D Williams

Abstract

A simple and versatile technique for the preparation of ultra-thin sections, which can be stained immunohistochemically directly on electron microscope grids, is presented. An anti-hapten immunoperoxidase procedure has been adapted for use on tissue fixed in a purified monomeric glutaraldehyde--picric acid mixture, and embedded in 'L R White', a recently formulated plastic resin. This plastic tolerates the use of partial dehydration of tissue, resulting in higher antigenic yields. In addition, no etching of ultra-thin sections is necessary, and the whole immunostaining procedure can be completed in less than 2 h. A comparison of commonly used fixatives is discussed. High-resolution micrographs showing general staining (uranyl acetate--lead citrate) of rat pancreas, and immunostaining of insulin and TSH in storage granules in perfusion-fixed rat tissue and of lambda-chain immunoreactive cells in immersion-fixed human tonsil are included as examples.

References

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Citations

Oct 1, 1994·Pflügers Archiv : European journal of physiology·C G WighamS A Hodson
Aug 1, 1991·The Histochemical Journal·G StranskyS Gay
Jun 1, 1987·The Histochemical Journal·A Tsukise, K Yamada
Jul 20, 2007·Archives of Dermatological Research·Tadashi YasuiAzuma Tsukise
Mar 20, 2012·Archives of Dermatological Research·Kousuke FukuiAzuma Tsukise
Aug 15, 2012·Archives of Dermatological Research·Takayuki NaraAzuma Tsukise
Jul 31, 2008·Histochemistry and Cell Biology·Srikanth Karnati, Eveline Baumgart-Vogt
Jun 8, 2013·Histochemistry and Cell Biology·Srikanth KarnatiEveline Baumgart-Vogt
Sep 19, 1991·Brain Research. Developmental Brain Research·T Nakayama, S Furuya

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