A simple procedure for morphometric analysis of processes and growth cones of neurons in culture using parameters derived from the contour and convex hull of the object

Journal of Neuroscience Methods
A KawaV Berezin

Abstract

Morphometric estimation of neuronal processes is currently laborious and time-consuming, since the individual processes (axons and dendrites) have to be traced manually. In order to facilitate the measurement of cellular processes, we have tested a series of parameters derived from the contour and the convex hull of an object and estimated to which extent they reflect process length and number. The parameters included the area, perimeter and form factor of the object and convex hull, their ratios as well as object length, breadth, width, length/width and spreading index. Some new parameters derived from the contour and convex hull of the object, were also computed: process index (the number of areas contained within the convex hull outside the object contour), process domain (the total area contained within the convex hull outside the object contour), their ratio and the square root of the process domain (SR process domain). In total, 18 parameters were estimated. Populations of motoneurons, growth cones of cerebellar granule cells and N2a neuroblastoma cells were utilized due to their diversity in morphological features. The processes of each object were drawn by hand to establish the actual length and number. Total process le...Continue Reading

References

Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I SchousboeA Schousboe
May 1, 1995·Journal of Neuroscience Methods·L de Medinaceli
Feb 1, 1993·Mechanisms of Ageing and Development·A Messina, C Bell
Dec 1, 1993·Computer Methods and Programs in Biomedicine·M MasseroliG Forloni

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Citations

Jul 16, 2002·Hearing Research·Dominik BrorsStefan Dazert
Nov 5, 2003·Molecular Biology of the Cell·Brian T HelfandRobert D Goldman
Mar 7, 2002·European Journal of Oral Sciences·Eugene LepekhinErik Dabelsteen
Mar 12, 2015·Journal of Tissue Engineering and Regenerative Medicine·Joana LoboAbhay Pandit
May 29, 2002·Toxicology and Applied Pharmacology·Peter S WalmodElisabeth Bock
Jun 1, 2005·Journal of Biomechanics·Iva Marija Tolić-Nørrelykke, Ning Wang
Jul 12, 2003·Pain·Viveca LindhStellan Håkansson
Sep 21, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Orit ShefiAmir Ayali
Jul 12, 2003·Journal of Biomolecular Screening·Peter RammBohdan J Soltys
Nov 10, 2001·Journal of Neurochemistry·E A LepekhinM Pekny
May 29, 2013·Advanced Healthcare Materials·Haiyang YuWinston Shim

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