PMID: 6985942Jan 1, 1980Paper

A simple procedure to use whole serum as a source of either IgG- or IgM-specific antibody

Journal of Immunological Methods
M D Boyle, J J Langone

Abstract

Absorption of rabbit antiserum to Forssman antigen with immobilized staphylococcal Protein A or concanavalin A selectively removed IgG or IgM antibodies, respectively. This absorption procedure was more rapid than ion exchange chromatography on DEAE cellulose or molecular sieving on Sephadex G-200 and gave a better yield of functionally purer antibody. This absorption method gave antiserum suitable for the preparation of either IgM or IgG sensitized sheep erythrocytes and should be of value for the large-scale preparation of indicator cells required for the study of complement action and for detection of specific receptors on cell surfaces.

Citations

Dec 30, 1982·Journal of Immunological Methods·J J Langone
Apr 15, 1983·Journal of Immunological Methods·K J ReisM D Boyle
Oct 25, 1991·Journal of Immunological Methods·A J GuidryD C Westhoff
Jan 1, 1985·Comparative Immunology, Microbiology and Infectious Diseases·M J LawmanM D Boyle
Sep 1, 1981·Molecular Immunology·T BorsosJ J Langone
Sep 1, 1985·Molecular Immunology·M D BoyleM J Lawman
Aug 1, 1986·Molecular Immunology·G O von Mering, M D Boyle
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M D Boyle, M Young
Sep 1, 1981·Clinical Immunology and Immunopathology·M D BoyleT Borsos
Jan 1, 1984·Annals of the New York Academy of Sciences·T EndoD M Marcus
Nov 1, 1986·Scandinavian Journal of Immunology·M Yarnall, M D Boyle
Jan 31, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·P ToulonJ Amiral
Nov 1, 1984·Scandinavian Journal of Immunology·K J ReisM D Boyle
Oct 1, 1985·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·R DeneckeG Trautwein
Oct 1, 1981·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·I J SeppäläV V Valtonen

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