PMID: 900853Jul 1, 1977Paper

A simple radioimmunoassay for plasma cortisol: comparison with the fluorimetric method of determination

Annals of Clinical Biochemistry
P J CarrH Crowley

Abstract

A quick and simple method for the radioimmunoassay of plasma cortisol is described. The mean morning plasma cortisol concentration in 43 normal subjects was 9.8 +/- 3.1 (S.D.) microgram/100 ml with a range of 5.0-19.5 microgram/100 ml. Mean midnight concentration in 24 normal subjects was 4.3 +/- 2.3 (S.D.) microgram/100 ml with a range of 1.4-9.6 microgram/100 ml. When compared with the fluorimetric method the mean results by radioimmunoassay of 154 routine specimens were 23% lower. In samples from unstimulated patients, regression analysis of results obtained by the two methods gave a correlation coefficient (r) of 0.93, regression line slope of 1.1, and intercept of 1.4 microgram. Mean radioimmunoassay results were 15% lower. When plasma cortisol concentration was above the normal range (greater than 30 microgram/100 ml) the regression line slope was 0.87, the intercept 17.9 microgram, r = 0.87 and mean radio immunoassay results were 37% lower. Plasma cortisol concentration in patients after insulin or Synacthen stimulation exhibited similar responses when measured by either method. Plasma cortisol concentration in normal subjects given metyrapone was lower when measured by radioimmunoassay (mean +/- S.D. = 8.7 +/- 2.7 micro...Continue Reading

References

Nov 1, 1975·The Journal of Clinical Endocrinology and Metabolism·W A Colburn
Aug 1, 1972·The Journal of Clinical Endocrinology and Metabolism·H J RuderM B Lipsett
Oct 1, 1966·The Journal of Clinical Endocrinology and Metabolism·C A Nugent, D M Mayes
Mar 1, 1963·The Journal of Clinical Endocrinology and Metabolism·B P MURPHYC J PATTEE
Mar 1, 1975·Annals of Clinical Biochemistry·M F CrowleyJ W Tuttlebee

❮ Previous
Next ❯

Citations

Apr 7, 2015·The Journal of Clinical Endocrinology and Metabolism·Leonie H A BroersenOlaf M Dekkers
Apr 1, 1990·The American Journal of Physiology·P W ClarkE W Kraegen
May 1, 1978·Annals of Clinical Biochemistry·R Morris
Nov 1, 1983·Annals of Clinical Biochemistry·R F ViningK Y Ho
Jul 1, 1983·The American Journal of Physiology·E W KraegenD J Chisholm
Sep 1, 1991·The American Journal of Physiology·S M FurlerE W Kraegen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry
T UmedaT Sato
Clinica Chimica Acta; International Journal of Clinical Chemistry
J Seth, L M Brown
Journal of Endocrinological Investigation
S AkgunG Schneider
© 2022 Meta ULC. All rights reserved