A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood

Experientia
F J LuW C Huang

Abstract

A simple, sensitive, non-stimulated assay was developed to measure the superoxide anion concentration in whole blood, using an ultra-sensitive chemiluminescence (CL) analyzer and lucigenin amplification. The assay system can be performed without leukocyte isolation or stimulant administration. The blood CL levels of healthy males (362.8 +/- 337.7 counts /10 sec) were not different from those of females (335 +/- 308.7 counts/10 sec) (p=0.64), whereas the CL levels in whole blood in patients with acute pancreatitis (2522 +/- 2014 counts/10 sec) were significantly higher than those of healthy controls (p<0.001). This assay system may be valuable in the future for quantitative measurement of reactive oxygen species in various disorders.

References

Jan 17, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·A NakagawaraS Minakami
Apr 1, 1992·American Journal of Clinical Pathology·M F Tosi, A Hamedani
Dec 1, 1992·Experientia·E M Lilius, P Marnila
Apr 1, 1992·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·V KaeverK Resch
Jan 1, 1991·Rheumatology International·D WendlingD A Vuitton
Jul 1, 1991·Journal of Molecular and Cellular Cardiology·K L Ver DonckH Van Belle
Sep 1, 1991·Reviews of Infectious Diseases·R W Steele
Apr 1, 1991·Biological Chemistry Hoppe-Seyler·L RoxvallM Heideman
Jan 1, 1989·Scandinavian Journal of Infectious Diseases·K PauksensP Venge
Apr 1, 1989·American Journal of Clinical Pathology·A TrulsonP Venge

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Citations

Oct 2, 2002·European Journal of Pharmacology·Yih-Loong Lai, Pei-chen Huang
Jul 24, 1998·Burns : Journal of the International Society for Burn Injuries·J S SunF J Lu
Aug 31, 2001·The American Journal of Chinese Medicine·C L HsiehC T Hsieh
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Upa KukongviriyapanSrisomporn Preeprame
Nov 30, 2007·Chemico-biological Interactions·Hui-Mei LinFen-Pi Chou
Jul 14, 2007·Clinical Biochemistry·Tze-Kiong ErTsan-Zon Liu
May 23, 2012·Phytotherapy Research : PTR·Hui-Fang ChiuChin-Kun Wang
Nov 7, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Saowanee LuangaramPatchareewan Pannangpetch
Jan 3, 2006·Colloids and Surfaces. B, Biointerfaces·F-C Kung, M-C Yang
Feb 2, 2018·Journal of Food and Drug Analysis·Jau-Tien LinDeng-Jye Yang
Nov 28, 2020·Andrologia·Anuson PoasakatePoungrat Pakdeechote
Feb 10, 2021·Antioxidants·Anuson PoasakatePoungrat Pakdeechote
Apr 24, 2001·Journal of the American Society of Nephrology : JASN·Chiang-Ting ChienSu-Ming Hsu

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