PMID: 8600976Mar 15, 1996Paper

A simple solid-state NMR method for determining peptidoglycan crosslinking in Bacillus subtilis

Biochimica Et Biophysica Acta
N S ShenoudaG E Wilson

Abstract

The crosslink index of the peptidoglycan of intact cell walls of Bacillus subtilis grown in media containing L-[15N]aspartic acid has been determined by a combination of cross-polarization magic-angle spinning 15N NMR and gas chromatography/mass spectrometry. The crosslink index of 62% decreased to 52% when the bacteria were exposed to the antibiotic cephalothin.

References

Nov 23, 1971·Biochemistry·A D Warth, J L Strominger

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Citations

Aug 24, 2013·PLoS Genetics·Coney Pei-Chen LinAaron M Neiman

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Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

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