PMID: 10838414Jun 6, 2000Paper

A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

Journal of Biomolecular Screening
J H ZhangK R Oldenburg

Abstract

The ability to identify active compounds (³hits²) from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the ³suitability² of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the quality of assays directly. In this report, a screening window coefficient, called ³Z-factor,² is defined. This coefficient is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment. The Z-factor is a dimensionless, simple statistical characteristic for each HTS assay. The Z-factor provides a useful tool for comparison and evaluation of the quality of assays, and can be utilized in assay optimization and validation.

References

Sep 20, 1995·Genomics·J R KorenbergJ C Venter
Jan 1, 1997·Nucleic Acids Research·K H FasmanD T Kingsbury

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Jan 18, 2012·Archives of Virology·Yu-Chen HsuHuey-Nan Wu
Feb 24, 2010·Amino Acids·Irina VetterGlenn F King
Sep 16, 2008·Annals of Biomedical Engineering·Alice H HuangRobert L Mauck
Jan 9, 2008·Apoptosis : an International Journal on Programmed Cell Death·G Wei XuAaron D Schimmer
Jan 25, 2008·Apoptosis : an International Journal on Programmed Cell Death·Jane ThompsonJohn Sharkey
Jun 29, 2007·Biotechnology Letters·Oliver J MillerPaul A Dalby
Apr 27, 2007·Investigational New Drugs·Pavel KrejciWilliam R Wilcox
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Jun 12, 2012·Molecular Biotechnology·Nunzianna DotiMenotti Ruvo
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Nov 5, 2003·Analytical Biochemistry·Jonathan WhitfieldJames M Staddon
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Jul 16, 2005·Analytical Biochemistry·Manjunath K RamaraoJames D Clark
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