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A simple strand-specific RNA-Seq library preparation protocol combining the Illumina TruSeq RNA and the dUTP methods

Biochemical and Biophysical Research Communications

Jun 15, 2012

Marc SultanMarie-Laure HA Yaspo

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Abstract

Preserving the original RNA orientation information in RNA-Sequencing (RNA-Seq) experiment is essential to the analysis and understanding of the complexity of mammalian transcriptomes. We describe herein a simple, robust, and time-effective protocol for generating strand-specific RNA-se...read more

Mentioned in this Paper

Deoxyuracil Nucleotides
Endoribonucleases
Reverse Transcription
Pancreatic ribonuclease
Ribonucleases
deoxyuridine triphosphate
Dilution Technique
Ribonuclease H
Cyclin D1
DNA ligase
Paper Details
References
    • References7
    • Citations28
    • References7
    • Citations28
  • A simple strand-specific RNA-Seq library preparation protocol combining the Illumina TruSeq RNA and the dUTP methods

    Biochemical and Biophysical Research Communications

    Jun 15, 2012

    Marc SultanMarie-Laure HA Yaspo

    PMID: 22609201

    DOI: 10.1016/j.bbrc.2012.05.043

    Abstract

    Preserving the original RNA orientation information in RNA-Sequencing (RNA-Seq) experiment is essential to the analysis and understanding of the complexity of mammalian transcriptomes. We describe herein a simple, robust, and time-effective protocol for generating strand-specific RNA-se...read more

    Mentioned in this Paper

    Deoxyuracil Nucleotides
    Endoribonucleases
    Reverse Transcription
    Pancreatic ribonuclease
    Ribonucleases
    Paper Details
    References
    • References7
    • Citations28
    • References7
    • Citations28
  • Get paper from

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