A simple strategy of probe DNA immobilization by diazotization-coupling on self-assembled 4-aminothiophenol for DNA electrochemical biosensor

Biosensors & Bioelectronics
Feng LiShusheng Zhang

Abstract

A novel and simple strategy for fabricating of DNA electrochemical biosensor was developed based on covalent coupling of probe NH(2)-ssDNA (S(1)) on Au electrode that had been functionalized by diazotization of assembled 4-aminothiophenol (4-ATP) monolayer. The thiol group of 4-ATP allowed the stable assembly of 4-ATP monolayer. The following diazotization reaction was directly performed to prepare functional diazo-ATP film for covalent coupling of probe S(1). Remarkably, it was noting that the diazo-ATP provided a surface with high conductibility for electron transfer. The complementary ssDNA was determined by using differential pulse voltammetry. The linear range of the developed biosensor was from 1.57 x 10(-9) to 4.52 x 10(-7)M with a detection limit of 3.26 x 10(-10)M. The fabricated biosensor possessed good selectivity and could be regenerated. The covalent immobilization of probe S(1) by simple diazotization-coupling on self-assembled 4-ATP monolayer could serve as a versatile platform for DNA immobilization and biosensors fabricating.

References

Feb 12, 2008·Analytica Chimica Acta·Fausto LucarelliMarco Mascini

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Citations

Feb 3, 2012·Analytical and Bioanalytical Chemistry·Patoommarn WipawakarnDanny K Y Wong
Dec 2, 2011·The Analyst·Zimple MatharuB D Malhotra
Jan 23, 2013·Analytical and Bioanalytical Chemistry·Mathieu Lazerges, Fethi Bedioui
May 31, 2017·Analytical and Bioanalytical Chemistry·Thu Huong HoMathieu Lazerges
Jun 1, 2011·Physical Chemistry Chemical Physics : PCCP·Masataka HakamadaMamoru Mabuchi
Sep 3, 2021·ACS Applied Materials & Interfaces·Zhiyang Zhang, Janina Kneipp

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