A simple technique for identification of "unreactive" light chains of immunoglobulins

Clinica Chimica Acta; International Journal of Clinical Chemistry
D Netto, A O Vladutiu

Abstract

Immunoelectrophoresis and immunofixation are not successful in determining the light chain type of some monoclonal immunoglobulins having configurational changes in their light chain structure ("unreactive" light chains) which prevent precipitation reaction with anit-light chain sera. An immunoselection technique was used to ascertain the light chain type of two such monoclonal immunoglobulins of IgD and IgA classes. It appears that a co-precipitation reaction in this assay circumvents the problem of "hidden" determinants. This technique is simple, reproducible, and could be used to characterize monoclonal immunoglobulins that do not precipitate with anit-light chain sera.

References

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Citations

Apr 20, 2013·Clinical Journal of the American Society of Nephrology : CJASN·Maria Teresa RocchettiLoreto Gesualdo
Jun 19, 2002·Blood·Andreas GoldammerMartin Haas
Jun 14, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Jeffrey M FaintStephen J Harding
Jan 31, 2018·Clinical Chemistry·David F Keren
Jul 17, 2003·European Journal of Clinical Investigation·G CohenW H Hörl
Oct 9, 2021·The Journal of Clinical Investigation·Kel Vin WooDavid M Ornitz

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