PMID: 6406769Apr 1, 1983Paper

A simple technique to study the effect of drugs on the negative inotropic response elicited by postganglionic cholinergic nerve stimulation in guinea pig atria

Journal of Pharmacological Methods
E Leung, F Mitchelson

Abstract

A simple technique is described for evaluating the effects of drugs on postganglionic cholinergic nerve stimulation in guinea pig atrial tissue. Raising the voltage of stimulation tenfold (high voltage stimulation, HVS) in the left atrium produced a positive inotropic response. Propranolol abolished this effect but failed to reveal a negative inotropic response. However, in atria obtained from 6-hydroxydopamine (6-OHDA) pretreated animals, HVS produced a negative inotropic effect. The magnitude of the response was frequency-dependent and was potentiated by the anticholinesterase dyflos. Tetrodotoxin abolished the response but mecamylamine had no significant effect. Drugs which inhibit cardiac muscarinic receptors caused a parallel shift of the frequency-response relationship. In the right atrium, HVS caused a biphasic inotropic response. Propranolol or 6-OHDA pretreatment inhibited the positive inotropic effect, thereby enhancing the negative inotropic response. The effect did not show as great a dependence on the frequency of stimulation as in the left atrium and was less useful for comparing the effectiveness of muscarinic receptor antagonists.

References

Nov 1, 1977·Clinical and Experimental Pharmacology & Physiology·F Mitchelson, R A O'Shea
Nov 15, 1978·European Journal of Pharmacology·J W Wei, P V Sulakhe
Mar 1, 1966·The Journal of Pharmacy and Pharmacology·T B Bolton, C Raper
Feb 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·H FuderE Muscholl
Jan 1, 1984·European Journal of Clinical Pharmacology·E Müller-Schweinitzer
May 7, 1982·European Journal of Pharmacology·E Leung, F Mitchelson
Oct 1, 1967·British Journal of Pharmacology and Chemotherapy·T B Bolton

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