PMID: 8609207Nov 1, 1995Paper

A simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukopenia virus in feces

Journal of Virological Methods
B SchunckUwe Truyen

Abstract

A polymerase chain reaction (PCR) assay is described for the detection of parvovirus in feces of dogs and cats. A touch-down protocol was used which enabled the specific amplification of virion DNA from feces after a fast and simple boiling pretreatment. The sensitivity of PCR was as high as ten infectious particles per reaction which corresponds to a titer of about 10(3) infectious particles per gram of unprocessed feces. This renders the PCR about 10- to 100-fold more sensitive than electron microscopy, the standard method for parvovirus diagnosis. The very rapid and simple sample preparation recommends this PCR assay as an alternative technique for routine parvovirus diagnosis.

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Citations

Jun 1, 2010·Indian Journal of Virology : an Official Organ of Indian Virological Society·S Nandi, Manoj Kumar
Feb 5, 2008·Journal of Feline Medicine and Surgery·Felix F NeuererKatrin Hartmann
Jun 2, 2009·Journal of Feline Medicine and Surgery·Uwe TruyenMarian C Horzinek
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