A SimpleProbe® real-time PCR assay for differentiating the canine parvovirus type 2 genotype

Journal of Clinical Laboratory Analysis
Minh HoangChao-Nan Lin

Abstract

Canine parvovirus type 2 (CPV-2) causes an important canine viral disease worldwide. CPV-2 belongs to the Protoparvovirus genus in the family Parvoviridae. An amino acid change at position 426 of the VP2 protein differentiate types of CPV-2, designated as CPV-2a (Asn), CPV-2b (Asp), and CPV-2c (Glu). In this study, we compared CPV-2 genotyping results obtained by SimpleProbe® real-time PCR and DNA sequencing analysis to identify the accuracy and sensitivity of these methods. One hundred rectal swabs were collected from CPV-2 naturally infected dogs from 2015 to 2017 at the Animal Disease Diagnostic Center, National Pingtung University of Science and Technology. CPV-2 genotyping was performed by SimpleProbe® real-time PCR and DNA sequencing to compare results. CPV-2a (n = 23), 2b (n = 6) and 2c (n = 71) genotyping results obtained by both techniques were identical with specificity of 100% for SimpleProbe® assay. In the SimpleProbe® assay, amplifying the DNAs prepared from the clinical specimens showed three distinct melting curve peaks. CPV-2b had the highest melting peak of 57.8°C (CI 95%: 57.7-58.5°C) followed by CPV-2c with a slightly lower melting peak of 52.3°C (CI 95%: 52.2-53.2°C) and CPV-2a with the lowest peak of 50.2°C...Continue Reading

References

Dec 1, 1991·Journal of Virology·C R ParrishH O Mohammed
Jan 1, 1995·Journal of Clinical Microbiology·M SendaO Itoh
Nov 21, 2001·The Journal of General Virology·C BuonavogliaL Carmichael
Oct 27, 2004·Archives of Virology·M NakamuraH Akashi
Apr 26, 2005·Journal of Virological Methods·Costantina DesarioCanio Buonavoglia
Nov 30, 2005·Journal of Virological Methods·Nicola DecaroCanio Buonavoglia
Feb 3, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sandra GiancolaDominique Brunel
Jun 13, 2006·Veterinary Microbiology·Uwe Truyen
Apr 25, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·K A NielsenS Gille
May 29, 2007·Veterinary Microbiology·Ruben PérezMartín Hernández
Sep 8, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Charles HongJeremiah T Saliki
Jun 26, 2009·Research in Veterinary Science·S NandiR S Chauhan
Oct 4, 2011·Veterinary Microbiology·Nicola Decaro, Canio Buonavoglia
Jan 28, 2012·Virus Research·Luciane Dubina PintoCláudio Wageck Canal
Jul 16, 2013·Veterinary Microbiology·Jaime AldazRuben Pérez
Dec 20, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·César Pedroza-RoldánMario Alberto López-Amezcua
Jul 21, 2015·Transboundary and Emerging Diseases·H ZhaoS Cheng
Mar 19, 2016·Journal of Virological Methods·Gurpreet KaurDeepti Narang
Sep 25, 2016·Virology Journal·Shu-Yun ChiangChao-Nan Lin
Dec 14, 2017·International Journal of Molecular Sciences·Yung-Cheng LinChao-Nan Lin

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Datasets Mentioned

BETA
AY869724

Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

SimpleProbe
Permatch

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