PMID: 1189529Jul 1, 1975Paper

[A simplified method for the fluorometric determination of 5-hydroxy-indoleacetic acid in the human liquor cerebrospinalis (author's transl)].

Zeitschrift Für Klinische Chemie Und Klinische Biochemie
F Geissbühler

Abstract

The fluorometric analysis of 5-hydroxy-indoleacetic acid by condensation with o-phthaldialdehyde has been modified by substituting a HCl-H2SO4 mixture at room temperature for hot concentrated HCl. The sensitivity of the method was increased by a factor of 2.7.

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