PMID: 6989507May 1, 1980Paper

A simplified method of DNA distribution analysis

Cell and Tissue Kinetics
P N Dean

Abstract

Current methods of analysing DNA distributions utilize complex mathematical expressions that require the use of large non-linear curve fitting methods and, consequently, large computers. This paper presents a new method of analysing DNA distributions of asynchronously growing or mildly perturbed cells. The S phase fraction is obtained by fitting a second degree polynomial to that part of the distribution, mid S phase, which is not influenced by either the G1 or the G2 + M peaks. The method is simple and fast, it exceeds the accuracy of other methods and it can be used on a large desk calculator or mini-computer.

References

Jun 1, 1976·Computers and Biomedical Research, an International Journal·J Fried
Feb 1, 1974·The Journal of Cell Biology·P N Dean, J H Jett

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Citations

Feb 16, 2002·Cell Biology International·Hideki KudoShinobu Sakamoto
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Feb 1, 1990·Experimental Cell Research·M Lalande

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