PMID: 7334942Jan 1, 1981Paper

A simplified micro-method for cytotoxicity testing using a flat-type titration plate for the detection of H-2 antigens

Microbiology and Immunology
T ShiroishiK Moriwaki

Abstract

A new micro-method for cytotoxicity testing using a flat-type titration plate has been developed. This micro-method is so simple that it involves no washing and staining procedures. In this method, cell viability is judged on the basis of the refractility change in the lymphocytes seen under the phase-contrast microscope. This micro-method has higher sensitivity and reproducibility than the dye exclusion method and 51Cr-release assay. Fixation of cells with glutaraldehyde and tight covering of the plate allow us to read the result even after 3 weeks. These advantage of the new method enable us to deal with a large number of samples at one time. We also introduce a rapid technique for the preparation of a cell suspension from the lymph node without killing the animal.

References

Dec 1, 1971·Transplantation·J H Pincus, R O Gordon
Mar 1, 1969·Transplantation·D B AmosA Tiilikainen
Dec 5, 1964·Nature·P I TERASAKI, J D MCCLELLAND
Dec 1, 1978·Immunogenetics·B M LongeneckerC Havele

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Citations

Dec 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M NishimuraH Hiai
Jun 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A FujimotoT Tomita
Jun 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T SakuraiM Noguchi
Jan 1, 1987·Immunogenetics·T ShiroishiK Moriwaki
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J D MarshallB Paigen
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T ShiroishiK Moriwaki
Mar 26, 2003·Cell Structure and Function·Zhi-ping ZongNobuo Yamaguchi
Nov 25, 1982·Nature·T ShiroishiK Moriwaki
Jul 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Bradford D HaskellEdward H Leiter

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