PMID: 9542650May 23, 1998Paper

A simplified model of hypoxic injury in primary cultured rat hepatocytes

In Vitro Cellular & Developmental Biology. Animal
T KamiyaY Kamiyama

Abstract

The Anaeropack system for cell culture, which was originally designed for the growth of anaerobic bacteria, was used to produce a hypoxic atmosphere for cultured hepatocytes. We measured changes in the oxygen and carbon dioxide concentrations and the atmospheric temperature in an airtight jar. We also measured changes in the pH of the medium during hypoxia to assess the accuracy of this system. Moreover, we used three durations (2, 3, and 4 h) of hypoxia and 8 h of reoxygenation in cultured rat hepatocytes, and then measured the lactate dehydrogenase (LDH), ketone body concentration (acetoacetate + beta-hydroxybutyrate), and the ketone body ratio (KBR: acetoacetate/beta-hydroxybutyrate) in the medium in order to assess the suitability of this system as a model for reperfusion following liver ischemia. The oxygen concentration dropped to 1% or less within 1 h. The concentration of carbon dioxide rose to about 5% at 30 min after the induction of the hypoxic conditions, and was maintained at this level for 5 h. No effect of the reaction heat produced by the oxygen absorbent in the jar was recognized. The extent of cell injury produced by changing the hypoxic parameters was satisfactorily reflected by the KBR, the ketone body conce...Continue Reading

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Citations

Jan 14, 2016·Chinese Journal of Integrative Medicine·Xiao-Qing ZhuRen-Bin Qi
May 13, 2008·International Journal of Cardiology·Hiroyuki AtsutaMasahiko Kurabayashi
May 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christophe CorpechotOlivier Rosmorduc
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T InoueT Okumura
Dec 7, 2007·Molecular Carcinogenesis·Hiromi SatoTomohiro Yano
Dec 6, 2019·Medical Molecular Morphology·Tomotake TabataYoichi Kondo
Nov 13, 2015·American Journal of Physiology. Cell Physiology·Hirobumi AsaiMie Watanabe-Akanuma
Jun 2, 2017·Experimental and Therapeutic Medicine·Zhixiong MeiJianhui Fan
Aug 1, 2019·Experimental and Therapeutic Medicine·Congying ZhaiYan Qu
Jan 10, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Heng ZhangYueping Shi

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