PMID: 108336Jan 1, 1979

A simplified procedure for measuring the class of anti-bacterial antibodies by mixed reverse passive antiglobulin haemagglutination (MRPAH)

Journal of Immunological Methods
F M EggertR R Coombs

Abstract

A modified procedure is described for performing the MRPAH (mixed reverse passive antiglobulin haemagglutination) reaction as a simple micro-method to measure the classes of bacterial antibodies. This 'bacterial dilution procedure' gave results closely correlated with those obtained by the 'serum (sample) dilution procedure' previously reported and with great economy of materials, labour and time. The method was used to investigate human serum antibodies to Br. abortus and S. enteritidis and serum and secretory antibodies to Strep. mutans. The good reproducibility of the MRPAH reaction was demonstrated by re-examining brucellosis sera tested one year previously. MRPAH was sufficiently sensitive to demonstrate the small amounts of IgG and IgM antibodies to Strep. mutans in human colostrum and early milk. A rise of antibody levels in the different immunoglobulin classes G, A and M was readily demonstrated in sera from individuals with salmonellosis.

References

Jan 1, 1976·Infection·M Majer
May 1, 1966·The Journal of Experimental Medicine·M AdinolfiJ M Rose
Apr 13, 1966·Virchows Archiv Für Pathologische Anatomie Und Physiologie Und Für Klinische Medizin·T De BritoD O Penna
Jan 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·A ZipurskyJ Bienenstock

Citations

Aug 1, 1984·Veterinary Research Communications·F A Clifton-Hadley
Jul 16, 1987·Journal of Immunological Methods·R R CoombsM P Cranage
Dec 6, 2003·Comparative Immunology, Microbiology and Infectious Diseases·J E Deakin, D W Cooper
Feb 1, 1987·Journal of Dental Research·F M EggertY C Tam
Apr 1, 1986·Annals of the Rheumatic Diseases·A C HanglowR R Coombs
Oct 1, 1981·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·L EdeboR R Coombs
Dec 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·K E Magnusson

Related Concepts

Metazoa
Anthropoidea
Antibodies, Bacterial
Brucella abortus bacterium
Colostrum
Dose-Response Relationship, Immunologic
Hemagglutination Test
Milk, Human
Salmonella enteritidis
Dall Sheep

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