Jul 1, 1977

A simplified procedure for the isolation of bile acids from serum based on a batch extraction with the non-ionic resin--Amberlite XAD-7

Annals of Clinical Biochemistry
S Barnes, A Chitranukroh

Abstract

A simple and reproducible method using the non-ionic resin, Amberlite XAD-7, for the isolation of bile acids from serum by a batch procedure is described. Recoveries were greater than 95% for the non-sulphated bile acids and greater than 70% for the sulphate esters of bile acids. By using 1 g of resin, recoveries were independent of the mass (0.1-5 mumol) of the bile acid present. Up to 35 samples a day can be extracted without requiring dedication of the operator. When serum extracts were analysed by the 3alpha-hydroxysteroid dehydrogenase procedure for estimation of bile acids, virtually all the background fluorescence was eliminated. These extracts were also suitable for gas liquid chromatography, thin layer chromatography, and radioimmunoassay.

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Mentioned in this Paper

Taurocholic Acid, Monolithium Salt
Bile Acid Measurement
Chromatography
Resins, Plant
Quenocol
3-Hydroxysteroid Dehydrogenases
Deoxycholic Acid, Sodium Salt, 12beta-Isomer
Cholic Acids
Esters
Radioimmunoassay

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