A simplified procedure for the physical development of the sulphide silver method to reveal synaptic zinc in combination with immunocytochemistry at light and electron microscopy

Journal of Neuroscience Methods
S De Biasi, C Bendotti

Abstract

The pool of zinc present in excitatory synaptic terminals in normal and pathological conditions (for instance the status epilepticus induced by kainic acid) can be stained by a silver sulphide method followed by physical development of the insoluble zinc-sulphide complexes. In this study we applied a previously described simple and rapid developing procedure that reveals synaptic zinc, to the study of normal and pathological hippocampi and combined it with pre and postembedding immunocytochemical methods to detect different antigens. Normal and kainic acid-treated rats were perfused with fixative solutions containing sodium sulphide and 50 microm-thick vibratome sections of the hippocampi were incubated in a commercial developing solution (IntenSE M, Amersham). The developed vibratome sections were then (1) mounted for light microscopy or osmicated and epon-embedded for electron microscopy; or (2) processed for the preembedding immunoenzymatic detection of various antigens (GABA, parvalbumin, calbindin) with light and electron microscopy. Thin sections from epon-embedded samples were also processed for the postembedding immunogold localization of glutamate. This very simple and rapid procedure gives rise to zinc-specific staini...Continue Reading

References

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Citations

Sep 1, 2001·Neuroscience·G DanscherH D Schrøder
Mar 10, 2010·Cerebral Cortex·Noritaka IchinoheKathleen S Rockland
Mar 10, 2007·The Journal of Comparative Neurology·Toshio MiyashitaKathleen S Rockland
May 24, 2000·Brain Research·S M JoG Danscher
Mar 6, 2002·Microscopy Research and Technique·Carlos López-GarcíaAsunción Molowny
Dec 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T AkagiT Hashikawa
Aug 26, 2006·The European Journal of Neuroscience·Noritaka IchinoheKathleen S Rockland
Jun 29, 2005·The Journal of Comparative Neurology·Noritaka Ichinohe, Kathleen S Rockland
May 19, 2005·Journal of Molecular Histology·Makoto KanedaTsutomu Hashikawa

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