PMID: 6162016Jan 1, 1981Paper

A simplified radioimmunological method for the determination of human beta-endorphin in cerebrospinal fluid

Journal of Neurology
H PrzuntekM Witteler

Abstract

Human beta-endorphin-like immunoreactive substances (beta h-EI) in human cerebrospinal fluid (CSF) were determined radioimmunologically. The cross reactivity of the antibodies to human beta-endorphin (beta h-E) amounted to 40% for human beta-lipotropin (beta h-LPH) whilst it was less than 1% for leu- and metenkephalin, alpha- and gamma-endorphin, fraction I and II [5], substance P and alpha-MSH. Prior to radioimmunological determination, an adsorbtion of beta h-EI from CSF with silicic acid was carried out and followed by a desorbtion, using a mixture of aceton/hydrochloric acid. This method was chosen because of the ratio of beta h-LPH to beta h-E in the desorbate can be shifted in favour of beta h-E owing to the variation in recoveries r (r beta h-LPH = 33%, r beta h-E = 64%). On the one hand, this enables a more specific determination of beta h-E and, on the other hand, and separation of any peptidase than may be present [9]. An adsorbtion/desorbtion of 2 ml CSF surfaces to prove the presence of 20-150 pg/ml (65-48 fmol/ml) of beta h-EI. The CSF of 28 patients with various neurological diseases was examined and 24 of them had concentrations of 20-70 pg/ml beta h-EI. The remaining four which had concentrations less than 20 pg...Continue Reading

References

Apr 11, 1977·Biochemical and Biophysical Research Communications·C H LiD Yamashiro
Jul 15, 1978·Lancet·W J JeffcoateG M Besser
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R E MainsN Ling
Jun 27, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·V HölltA Herz
Jul 11, 1977·Biochemical and Biophysical Research Communications·R GuilleminT Vargo

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