A simplified routine assay for urinary 3-methoxy-4-hydroxyphenylglycol

Journal of Neural Transmission
J K YaoD P van Kammen

Abstract

Urinary 3-methoxy-4-hydroxyphenylglycol (MHPG) level may provide us with a biochemical index for central noradrenergic activity. Previous methods for assaying MHPG in urine often lacked specificity, sensitivity, cost-effectiveness or convenience. We now describe a simplified routine assay for urinary MHPG by high-pressure liquid chromatography using electrochemical detection. For convenience and cost-effectiveness within a typical batch assay of total MHPG, 0.5 mL of urine sample, 50 microL of glusulase and 4 micrograms iso-vanillyl alcohol (internal standard) were used to hydrolyze conjugated MHPG at 37 degrees C overnight. Alternatively, for a same-day operation, it is efficient to hydrolyze the sample at 50 degrees C for 3 hours. Each sample was separated isocratically on a reversed-phase column (Ultracarb 5 ODS) at 25 degrees C with the flow rate at 1 mL/min. Intra- and inter-assay coefficients of variation were found to be 4.0% (n = 10) and 5.0% (n = 27), respectively, for MHPG at a mean concentration of 1.9 mg/L. Sulfatase or glucuronidase can be substituted for glusulase to obtain either sulfate- or glucuronide-conjugated MHPG. This procedure requires smaller sample amounts and less preparation time without compromising ...Continue Reading

References

Jan 1, 1990·Journal of Neural Transmission. General Section·L Peyrin
Aug 1, 1974·Journal of Neurochemistry·A Foldes, J L Meek
Mar 1, 1972·Archives of General Psychiatry·J W MaasH Dekirmenjian
Jan 1, 1984·Journal of Neurochemistry·G ReinM Sandler
Jun 1, 1980·Journal of Neurochemistry·K J RenskersJ A Roth
May 1, 1982·Archives of General Psychiatry·M LinnoilaW Z Potter
Oct 1, 1980·Archives of General Psychiatry·P A BlomberyM H Ebert

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Citations

Feb 15, 2011·Neuroscience and Biobehavioral Reviews·Arne Dietrich, Michel Audiffren
Sep 16, 2011·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Giuseppe GuglielmiAlberto Bazzocchi

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