A simplified technique for producing an ischemic wound model.

Journal of Visualized Experiments : JoVE
Sufan Chien, Bradon J Wilhelmi

Abstract

One major obstacle in current diabetic wound research is a lack of an ischemic wound model that can be safely used in diabetic animals. Drugs that work well in non-ischemic wounds may not work in human diabetic wounds because vasculopathy is one major factor that hinders healing of these wounds. We published an article in 2007 describing a rabbit ear ischemic wound model created by a minimally invasive surgical technique. Since then, we have further simplified the procedure for easier operation. On one ear, three small skin incisions were made on the vascular pedicles, 1-2 cm from the ear base. The central artery was ligated and cut along with the nerve. The whole cranial bundle was cut and ligated, leaving only the caudal branch intact. A circumferential subcutaneous tunnel was made through the incisions, to cut subcutaneous tissues, muscles, nerves, and small vessels. The other ear was used as a non-ischemic control. Four wounds were made on the ventral side of each ear. This technique produces 4 ischemic wounds and 4 non-ischemic wounds in one animal for paired comparisons. After surgery, the ischemic ear was cool and cyanotic, and showed reduced movement and a lack of pulse in the ear artery. Skin temperature of the ischemi...Continue Reading

Citations

Jun 13, 2013·Journal of Vascular Surgery·Leena Pradhan NabzdykAristidis Veves
Mar 28, 2018·International Journal of Molecular Sciences·Matthew CaleyPhil Stephens
Jul 29, 2020·International Journal of Molecular Sciences·Elena GroppaSerena Zacchigna

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