A simulation and estimation framework for intracellular dynamics and trafficking in video-microscopy and fluorescence imagery

Medical Image Analysis
Jérôme BoulangerPatrick Bouthemy

Abstract

Image sequence analysis in video-microscopy has now gained importance since molecular biology is presently having a profound impact on the way research is being conducted in medicine. However, image processing techniques that are currently used for modeling intracellular dynamics, are still relatively crude and yield imprecise results. Indeed, complex interactions between a large number of small moving particles in a complex scene cannot be easily modeled, limiting the performance of object detection and tracking algorithms. This motivates our present research effort which is to develop a general estimation/simulation framework able to produce image sequences showing small moving spots in interaction, with variable velocities, and corresponding to intracellular dynamics and trafficking in biology. It is now well established that spot/object trajectories can play a role in the analysis of living cell dynamics and simulating realistic image sequences is then of major importance. We demonstrate the potential of the proposed simulation/estimation framework in experiments, and show that this approach can also be used to evaluate the performance of object detection/tracking algorithms in video-microscopy and fluorescence imagery.

References

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Citations

May 21, 2010·Annual Review of Biomedical Engineering·Jens Rittscher
Oct 12, 2010·Biomedical Engineering Online·Nezamoddin N KachouieEric Jervis
Oct 21, 2015·The Journal of Cell Biology·Hui-Ju YangYasushi Hiraoka
Dec 23, 2014·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Thierry PécotCharles Kervrann
Sep 19, 2012·Cellular Microbiology·Christophe Zimmer
Feb 15, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ge Yang
Sep 10, 2015·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Antoine BassetCharles Kervrann
Jun 27, 2009·IEEE Transactions on Medical Imaging·Ihor SmalErik Meijering
Dec 14, 2011·Computational and Mathematical Methods in Medicine·Chunyan YaoHouxiang Zhang
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Mar 2, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Şükriye BilirTokuko Haraguchi
Mar 5, 2013·Nature·Maho HamasakiTamotsu Yoshimori
Jun 24, 2014·Cell Structure and Function·Yuji ChikashigeYasushi Hiraoka
Apr 3, 2010·Cold Spring Harbor Protocols·Richard M PartonIlan Davis

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