PMID: 6161115Mar 1, 1980Paper

A simultaneous-coupling azo dye method for the quantitative assay of esterase using alpha-naphthyl acetate as substrate

The Histochemical Journal
K J Johnston, A E Ashford

Abstract

This paper describes a simultaneous-coupling azo dye method for the measurement of esterase activity using the histochemical substrate, alpha-naphthyl acetate. By the choice of two diazonium salts with optimal coupling characteristics, the reaction can be carried out at any pH between 3.0 and 9.5. The azo dye is maintained in solution for spectrophotometric measurements with bovine serum albumin. The simultaneous-coupling method is compared with an assay based on the direct measurement of released alpha-naphthol by its ultra-violet absorbance in a pH study of hog liver esterase. There is good agreement between the data obtained by both methods.

References

Jul 1, 1970·The Histochemical Journal·A Jarrett, N W Please
Jun 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Burline, L Galzigna
Jan 1, 1956·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M RUTENBURG, A M SELIGMAN
May 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V K HOPSU, P J MCMILLAN
Jan 1, 1950·The Journal of Clinical Investigation·A M SELIGMAN, M M NACHLAS
Jul 1, 1968·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G A Gall, W H Kyle

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Citations

Feb 1, 1983·The Histochemical Journal·H Luppa, J Andrä
Feb 1, 1990·The Histochemical Journal·S KirkebyT Zelander
Feb 27, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J SulaR Zemek
Jul 19, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Youji Shimazaki, Shizuka Miki
Oct 1, 2003·Archives of Insect Biochemistry and Physiology·Xiaodun He

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