A single adenovirus-mediated relaxin delivery attenuates established liver fibrosis in rats

The Journal of Gene Medicine
Ja Kyung KimKwan Sik Lee

Abstract

Liver fibrosis is characterized by excess accumulation and repressed degradation of extracellular matrix. While means of alleviating already established liver fibrosis has scarcely been reported, continuous relaxin (RLX) infusion has shown some promising results. In this study, we investigated whether a single adenoviral delivery of RLX would attenuate the established liver fibrosis in rats. Rats were given thioacetamide (TAA) for 8 weeks and infected once with either RLX-expressing adenovirus (TAA+RLX) or control virus (TAA+Vector) via tail vein. They were sacrificed either 3 days or 3 weeks after the adenovirus infection. Morphometric analysis of picrosirius red stained area demonstrated that TAA+RLX group had significantly decreased fibrosis at week 3 when liver fibrosis of TAA+Vector group remained unchanged. While the liver and serum RLX levels were elevated on day 3 and reversed by week 3, expression of RLX receptor (Rxfp1, Relaxin-like family peptide receptor-1), in TAA+RLX rats was sustained to be elevated. The production of tissue cyclic adenosine monophosphate, which is a second messenger of activated Rxfp1, was still enhanced in TAA+RLX group by week 3. Expression of lysyl oxidase homolog 2, which contributes in coll...Continue Reading

References

Aug 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R OrenZ Halpern
Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G Svegliati BaroniA Benedetti
Apr 3, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·L I Smith-Mungo, H M Kagan
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·M M BurcinB W O'Malley
Oct 12, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·E H KerkvlietV Everts
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R F SchwabeD A Brenner
Oct 5, 2001·Seminars in Liver Disease·D SchuppanE G Hahn
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 22, 2002·Cancer Gene Therapy·Jaesung KimChae-Ok Yun
Sep 2, 2003·Biochemical Pharmacology·Robert G BennettDean J Tuma
Apr 15, 2004·Endocrine Reviews·O David Sherwood
Apr 21, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Andrea GalliAlessandro Casini
Oct 19, 2006·Journal of the National Cancer Institute·Joo-Hang KimChae-Ok Yun
Oct 27, 2007·Molecular and Cellular Endocrinology·R A D BathgateX-J Du
Apr 5, 2008·Endocrinology·Chrishan S SamuelDarren J Kelly
May 13, 2008·Gastroenterology·Scott L Friedman
Jun 1, 2011·The British Journal of Dermatology·W J LeeC-O Yun
Jan 17, 2012·Journal of Hepatology·Elizabeth L Ellis, Derek A Mann
Jul 23, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Robert G BennettDean J Tuma
Jul 23, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jonathan A FallowfieldJohn P Iredale
Dec 3, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Young-Mi KangSeong-Hwan Moon
Feb 26, 2014·Nature Reviews. Immunology·Antonella PellicoroJonathan A Fallowfield

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Citations

Mar 19, 2014·PloS One·Malihe-Sadat Poormasjedi-MeibodAziz Ghahary
Jan 27, 2021·Nature Nanotechnology·Jonathan A Fallowfield, Prakash Ramachandran
Jun 9, 2021·Biochimie·Devaraj Ezhilarasan

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