PMID: 2122454Oct 1, 1990Paper

A single amino acid substitution in the variable region of the light chain specifically blocks immunoglobulin secretion

Proceedings of the National Academy of Sciences of the United States of America
J L Dul, Y Argon

Abstract

Although immunoglobulin light chains are usually secreted in association with heavy chains, free light chains can be secreted by lymphocytes. To identify the structural features of light chains that are essential for their secretion, we mutated a conserved sequence in the variable domain of a lambda I light chain. The effects of the mutations on secretion were assayed by transient expression in COS-1 cells. One mutant (AV60), which replaced Ala-60 with Val, was secreted as efficiently as wild-type lambda I by transfected COS-1 cells. This result was not surprising because secreted lambda II chains contain valine in this position. However, a second lambda I mutant (AV60FS62), which replaced Phe-62 with Ser as well as Ala-60 with Val, was not secreted. This mutant was arrested in the endoplasmic reticulum, as judged by immunofluorescence and by its association with a lumenal endoplasmic reticulum protein, immunoglobulin heavy chain binding protein (BiP). The defect in secretion was not due to gross misfolding of the lambda I chain, since cells cotransfected with AV60FS62 and an immunoglobulin heavy chain gene produced functional antigen-binding antibodies. These assembled IgM molecules were still not secreted. Hence, the replacem...Continue Reading

References

Aug 1, 1979·Journal of Virology·A ShieldsD Baltimore
Jul 1, 1978·The Journal of Experimental Medicine·G E SonensheinM L Gefter
Jun 1, 1979·European Journal of Immunology·S L Morrison, M D Scharff
Nov 1, 1979·The Journal of Cell Biology·A Tartakoff, P Vassalli
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·C MilsteinC D Wilde
May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Oct 1, 1986·The Journal of Cell Biology·C S CopelandA Helenius
Jan 1, 1989·Annual Review of Cell Biology·H R Pelham
Aug 20, 1986·Journal of Molecular Biology·Y SatowD R Davies
Dec 5, 1985·Journal of Molecular Biology·C ChothiaM Karplus
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M SegalD R Davies
Nov 11, 1983·Nucleic Acids Research·C M GormanR Reeves
Jan 1, 1983·Annual Review of Immunology·D R Davies, H Metzger
Apr 1, 1983·European Journal of Immunology·Y ArgonC Milstein
Nov 24, 1983·Nature·I G Haas, M Wabl

❮ Previous
Next ❯

Citations

May 1, 1995·Immunology Today·J Melnick, Y Argon
Oct 1, 1995·Current Opinion in Biotechnology·J J TrillS Ganguly
Oct 8, 2003·Journal of Molecular Biology·Jorge SepúlvedaOscar R Burrone
Sep 29, 1999·Immunology Today·F J Stevens, Y Argon
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W A MarascoS Y Chen
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·L HendershotY Argon
Sep 5, 2008·The Journal of Biological Chemistry·Elizabeth M BadenMarina Ramirez-Alvarado
May 29, 1998·Immunological Reviews·G D WiensM B Rittenberg
Jan 1, 1997·Annual Review of Microbiology·I J Rondon, W A Marasco
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M H SkowronekI G Haas
May 1, 1995·Immunotechnology : an International Journal of Immunological Engineering·W A Marasco
Jan 1, 1992·Immunopharmacology and Immunotoxicology·T V Tittle, B A Schaumann
Jul 16, 2011·American Journal of Clinical Pathology·Robert B LorsbachFalko Fend
Apr 3, 2012·Scientific Reports·Alicia D HennMartin S Zand
Feb 2, 2006·Journal of Biotechnology·Willemien H WielandDiego V Orzáez
Sep 23, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Shaji KumarParameswaran Hari
Apr 26, 2005·Methods : a Companion to Methods in Enzymology·Naoya TsurushitaShankar Kumar
Jun 21, 2017·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Elena S KlimtchukLawreen H Connors
May 25, 2004·Journal of Leukocyte Biology·Catherine DecourtMichel Cogné
Dec 25, 2010·Ergonomics·Jan Dul, Canan Ceylan
Feb 12, 2005·The Journal of Biological Chemistry·Yechiel ElkabetzShoshana Bar-Nun
Apr 20, 2004·Blood·Daniel CoriuAlan Solomon
Nov 17, 1995·The Journal of Biological Chemistry·G KnarrJ Buchner
Oct 9, 1999·The Journal of Biological Chemistry·G KnarrJ Buchner
Oct 12, 2007·Clinical and Vaccine Immunology : CVI·Odd OdinsenJean-Pierre Allain
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elizabeth A WhitcombMarvin B Rittenberg
Apr 21, 2020·Annals of Hematology·Magdalini MigkouMeletios A Dimopoulos
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G D WiensM B Rittenberg
Jun 11, 2021·Biotechnology and Bioengineering·Cai GuoRené Hubert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.