A single and rapid calcium wave at egg activation in Drosophila

Biology Open
Anna H York-AndersenTimothy T Weil

Abstract

Activation is an essential process that accompanies fertilisation in all animals and heralds major cellular changes, most notably, resumption of the cell cycle. While activation involves wave-like oscillations in intracellular Ca(2+) concentration in mammals, ascidians and polychaete worms and a single Ca(2+) peak in fish and frogs, in insects, such as Drosophila, to date, it has not been shown what changes in intracellular Ca(2+) levels occur. Here, we utilise ratiometric imaging of Ca(2+) indicator dyes and genetically encoded Ca(2+) indicator proteins to identify and characterise a single, rapid, transient wave of Ca(2+) in the Drosophila egg at activation. Using genetic tools, physical manipulation and pharmacological treatments we demonstrate that the propagation of the Ca(2+) wave requires an intact actin cytoskeleton and an increase in intracellular Ca(2+) can be uncoupled from egg swelling, but not from progression of the cell cycle. We further show that mechanical pressure alone is not sufficient to initiate a Ca(2+) wave. We also find that processing bodies, sites of mRNA decay and translational regulation, become dispersed following the Ca(2+) transient. Based on this data we propose the following model for egg activ...Continue Reading

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Citations

Aug 8, 2015·Open Biology·Benjamin LoppinBéatrice Horard
Mar 13, 2016·Molecular Reproduction and Development·Amber R KrauchunasAndrew Singson
Oct 26, 2016·Molecular Reproduction and Development·Ethan C Degner, Laura C Harrington
Dec 6, 2018·Molecular Reproduction and Development·Elle C Roberson, Elizabeth Ann L Enninga
Nov 28, 2018·Molecular & Cellular Proteomics : MCP·Zijing ZhangMariana F Wolfner
Aug 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Qinan Hu, Mariana F Wolfner
Dec 28, 2019·Molecular Reproduction and Development·Anna H York-AndersenTimothy T Weil
Jun 24, 2020·Emerging Topics in Life Sciences·M Sankaranarayanan, Timothy T Weil
Aug 1, 2020·Molecular Reproduction and Development·Qinan Hu, Mariana F Wolfner
Sep 4, 2015·Physical Biology·Cody NarcisoJeremiah Zartman
Jul 3, 2020·IScience·Qinan HuMariana F Wolfner
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Zijing ZhangMariana F Wolfner
Jan 10, 2020·Molecular Reproduction and Development·Elle C Roberson, Elizabeth Ann L Enninga
Aug 4, 2021·Open Biology·Anna H York-AndersenTimothy T Weil

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Fiji J

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