A single anti-microRNA antisense oligodeoxyribonucleotide (AMO) targeting multiple microRNAs offers an improved approach for microRNA interference.

Nucleic Acids Research
Yanjie LuBaofeng Yang

Abstract

Anti-miRNA antisense inhibitors (AMOs) have demonstrated their utility in miRNA research and potential in miRNA therapy. Here we report a modified AMO approach in which multiple antisense units are engineered into a single unit that is able to simultaneously silence multiple-target miRNAs, the multiple-target AMO or MTg-AMO. We validated the technique with two separate MTg-AMOs: anti-miR-21/anti-miR-155/anti-miR-17-5p and anti-miR-1/anti-miR-133. We first verified the ability of the MTg-AMOs to antagonize the repressive actions of their target miRNAs using luciferase reporter activity assays and to specifically knock down the levels of their target miRNAs using real-time RT-PCR methods. We then used the MTg-AMO approach to identify several tumor suppressors-TGFBI, APC and BCL2L11 as the target genes for oncogenic miR-21, miR-155 and miR-17-5p, respectively, and two cardiac ion channel genes HCN2 (encoding a subunit of cardiac pacemaker channel) and CACNA1C (encoding the alpha-subunit of cardiac L-type Ca(2+) channel) for the muscle-specific miR-1 and miR-133. We further demonstrated that the MTg-AMO targeting miR-21, miR-155 and miR-17-5p produced a greater inhibitory effect on cancer cell growth, compared with the regular sing...Continue Reading

References

Dec 22, 1999·Journal of Molecular Biology·V Patzel, G Sczakiel
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Aug 6, 2005·Science·Ramesh S PillaiWitold Filipowicz
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
Oct 15, 2005·Development·Ines Alvarez-Garcia, Eric A Miska
Nov 1, 2005·Nature·Jan KrützfeldtMarkus Stoffel
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Feb 14, 2006·Trends in Molecular Medicine·Scott M Hammond
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ligang WuJoel G Belasco
Jul 4, 2006·Genes & Development·Isabelle Behm-AnsmantElisa Izaurralde
Oct 31, 2006·Oncogene·M-L SiY-Y Mo
Nov 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eva van RooijEric N Olson
Jan 20, 2007·Circulation Research·Danish SayedMaha Abdellatif
Mar 9, 2007·The Journal of Biological Chemistry·Jiening XiaoZhiguo Wang
May 1, 2007·Nature Medicine·Alessandra CarèGianluigi Condorelli
May 15, 2007·Journal of Molecular and Cellular Cardiology·Mariko TatsuguchiDa-Zhi Wang
May 26, 2007·The American Journal of Pathology·Yunhui ChengChunxiang Zhang
Jul 4, 2007·Circulation·Thomas ThumJohann Bauersachs
Aug 2, 2007·Expert Opinion on Therapeutic Targets·Kandiah JeyaseelanArunmozhiarasi Armugam
Aug 2, 2007·Expert Opinion on Biological Therapy·Fritz Eckstein
Dec 18, 2007·Expert Opinion on Biological Therapy·Jan Stenvang, Sakari Kauppinen
Feb 27, 2008·Journal of Internal Medicine·T Dalmay
Apr 17, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Zhiguo WangBaofeng Yang

❮ Previous
Next ❯

Citations

Sep 28, 2010·Biotechnology Letters·Niall BarronMartin Clynes
Jun 30, 2011·Current Heart Failure Reports·Michael V G Latronico, Gianlugi Condorelli
Dec 8, 2009·Acta Pharmacologica Sinica·Zhen-wei PanBao-feng Yang
Nov 6, 2010·Cardiovascular Research·Zhiguo WangBaofeng Yang
Feb 2, 2010·European Heart Journal·Gianluigi CondorelliGerald W Dorn
Sep 24, 2009·Human Reproduction Update·E Maria C Ohlsson TeagueM Louise Hull
Jun 1, 2012·The Cancer Journal·Christopher J Cheng, Frank J Slack
Nov 30, 2011·Epigenomics·Erik D WiklundSusan J Clark
Sep 17, 2011·Journal of Nucleic Acids·Andrius ServaVytaute Starkuviene
Apr 20, 2014·International Journal of Genomics·Kalina Andreeva, Nigel G F Cooper
Apr 22, 2014·Molecular and Cellular Endocrinology·Marta LlauradóMarina Rigau
Jun 2, 2011·Journal of Aging Research·Andrea FelicianoMatilde E Lleonart
Dec 10, 2014·Nanomedicine·Huricha Baigude, Tariq M Rana
May 14, 2014·BioMed Research International·Kouji BannoDaisuke Aoki
Feb 24, 2010·Cardiovascular Research·Wolfgang PollerHenry Fechner
Jun 20, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sonia A Melo, Raghu Kalluri
Jan 24, 2013·Leukemia & Lymphoma·Simon HusbyKirsten Grønbæk
Aug 14, 2009·Expert Review of Cardiovascular Therapy·Sabine Telemaque, James D Marsh
May 9, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tarek ShalabyMichael A Grotzer
Jan 9, 2015·Expert Opinion on Investigational Drugs·Karen A ScottJohn F Cryan
Nov 19, 2014·Expert Opinion on Drug Discovery·Kristyn Campbell, Stephanie A Booth
Feb 5, 2016·Experimental Cell Research·Jinjun QianWei Xie
Dec 3, 2014·Pharmacology & Therapeutics·Brian S ComerWilliam T Gerthoffer
Jan 16, 2016·Journal of the American Chemical Society·Jaeyoung PaiInjae Shin
May 23, 2012·Trends in Pharmacological Sciences·Henriett ButzAttila Patócs
Jan 31, 2012·Biotechnology Advances·Tomaž BratkovičBoris Rogelj
May 11, 2011·Biochemical and Biophysical Research Communications·Jong-Joo LeeHyoung-Pyo Kim
Feb 16, 2016·Biochimica Et Biophysica Acta·Alessio Peracchi
Jan 10, 2012·The Journal of Gene Medicine·Chan Il ChangDong-Ki Lee
Jul 28, 2011·Medicinal Research Reviews·Jose A LealMatilde E Lleonart
Dec 15, 2012·Journal of Ovarian Research·Mohd Saif ZamanSubhash C Chauhan
Jul 3, 2015·Cardiology in Review·Nikolaos PapageorgiouDimitris Tousoulis
Jun 16, 2012·Artificial DNA, PNA & XNA·Concetta AvitabileAlessandra Romanelli
Oct 23, 2013·Molecular and Cellular Endocrinology·Guidong YaoFei Sun
Dec 5, 2014·Genes & Diseases·Colles Price, Jianjun Chen
Sep 23, 2014·Journal of Pediatric Gastroenterology and Nutrition·Rui DongShan Zheng

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
Protein Assay
transfections

Software Mentioned

QuantityOne
GraphPad Prism
Excel
IDT

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.